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Rabbit monoclonal gfp antibody

Manufactured by Abcam
Sourced in United Kingdom, United States

Rabbit monoclonal GFP antibody is a laboratory reagent used to detect and quantify green fluorescent protein (GFP) in biological samples. It is a highly specific and sensitive antibody raised in rabbits against GFP.

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2 protocols using rabbit monoclonal gfp antibody

1

Western Blot Analysis of Viral Proteins

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Cells were lysed in RIPA lysis buffer [150 mM NaCl, 1% Non-ionic detergent, 1% Sodium deoxycholate, 0.1%SDS, (Biomax, Republic of Korea)]. About 20 μg of proteins per sample were separated by SDS-PAGE using a 10%polyacrylamide gels and transferred to nitrocellulose membranes (Cytiva, USA). Membranes were blocked with 10% (w/v) non-fat dry milk in TBST buffer [100 mM Tris (pH 7.6), 150 mM NaCl, 0.1% (w/v) Tween-20] for 1 h at room temperature. Membranes were incubated with a 1:1,000 dilution of rabbit polyclonal anti-Gag antibody, 1:500 dilution of rabbit polyclonal anti-Env antibody, 1:5,000 dilution of rabbit monoclonal GFP antibody (Abcam, UK), and 1:5,000 dilution of rabbit monoclonal β-actin antibody (Cell Signaling Technology, USA) at 4°C overnight. The membranes were then incubated with a 1:10,000 dilution of secondary antibody, goat anti-rabbit IgG conjugated to HRP (BioRad, USA), for 1 h at room temperature. The chemiluminescent signal was visualized by exposing the blots to a chemiluminescence imaging system, E-blot (e-BLOT Life Science, China). Rabbit polyclonal anti-Env antibody was designed to be specific for PFV Env-SU domain and was custom-produced by AbClon (Korea).
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2

Western Blotting of ILK, GFP, and GAPDH

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Western blotting was conducted as previously described (14 (link)). Rabbit monoclonal ILK antibody (dilution, 1:500; cat. no. ab52480; Abcam, Cambridge, MA, USA), rabbit monoclonal GFP antibody (dilution, 1:1,000; cat. no. 2037S) and rabbit monoclonal GAPDH antibody (dilution, 1:1,000; cat. no. 3683) (both from CST, Beverly, MA, USA) were used. Protein band densities and were calculated by ImageJ followed by normalization using GAPDH as the internal control.
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