Primescript rt reagent kits with gdna erase

Total RNA was prepared from the fetal gonads of mutant embryos and the gonads of ICR strain embryos at each embryonic stage using RNeasy Mini Kits (Qiagen). Total RNA was then used for cDNA synthesis using PrimeScript RT Reagent Kits with gDNA Erase (Takara). PCR was performed with KAPA SYBR FAST qPCR Kits using a thermal cycler dice real time system (Takara). The primer pairs used for PCR amplification are listed in S3 Table.

Total RNAs were isolated from whole gonads of wild-type and BAC transgenic mice at each stage by RNeasy Mini Kit (Qiagen). One hundred ng (1W to 5W) and 40 ng (E12.5 to P0) of total RNA were used for cDNA synthesis using Prime Script RT Reagent Kits with gDNA Erase according to the manufacturer’s protocol (Takara). Real time PCR was performed with KAPA SYBR FAST qPCR kits using a thermal cycle dice real time system (Takara). The obtained data was normalized by Mvh.
The following primers were used for PCR amplification:
Dazl
Forward: 5′−CACGCCTCAGTGACTCGGCGAC−3’
Reverse: 5′−CGAAGCATACAGACAGTGGTC−3’
Mvh
Forward: 5′−GTTGAAGTATCTGGACATGATGCAC−3’
Reverse: 5′−CGAGTTGGTGCTACAATAATACACTC−3’
G3pdh
Forward: 5′−ACCACAGTCCATGCCATCAC−3’
Reverse: 5′−TCCACCACCCTGTTGCTGTA−3’
FLAG tagged DazlForward: 5′−CACGCCTCAGTGACTCGGCGAC−3’
Reverse: 5′−CACCGTCATGGTCTTGTAGTC−3’
Dazl cKO
Forward: 5′−GACTTACATGCAGCCTCCAACCATG−3’
Reverse: 5′−AACAGGCAGCTGATATCCAGTGATG−3’