High glucose

Manufactured by Corning

Sourced in United States

High glucose is a lab equipment product designed for use in scientific research and testing. It serves as a standard solution for calibrating and verifying the accuracy of glucose measurement devices and assays.

Automatically generated - may contain errors

Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) Hepatocyte media, by Thermo Fisher Scientific (2 mentions) Hydrocortisone, by Merck Group (2 mentions) Lewis rats, by Charles River Laboratories (2 mentions) Insulin, by Eli Lilly (2 mentions) Dulbecco modified eagle medium (dmem), by Corning (2 mentions) Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions) Antibiotic antimycotic, by Merck Group (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Cell titer aqueous one solution, by Promega (1 mentions) Hct116, by Thermo Fisher Scientific (1 mentions) Mccoy s 5a medium, by Thermo Fisher Scientific (1 mentions) Dmem f12 medium, by Thermo Fisher Scientific (1 mentions) Hct116, by American Type Culture Collection (1 mentions)

5 protocols using high glucose

Isolation and Culture of Rat Hepatocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rat hepatocytes were isolated from 2‐ to 3‐month old female Lewis rats (Charles River) by collagenase perfusion as described previously.28, 29, 30 Briefly, animals were anesthetized and the portal vein was cannulated. The liver was then perfused with buffers and digested with collagenase. The resultant digest was purified using Percoll centrifugation. Hepatocytes were cultured in DMEM with high glucose (Cellgro), 10% (v/v) fetal bovine serum (Gibco), 0.5 U mL⁻¹ insulin (Lilly), 7 ng mL⁻¹ glucagons (Bedford Laboratories), 7.5 µg mL⁻¹ hydrocortisone (Sigma), and 1% penicillin‐streptomycin (“Hepatocyte media,” Invitrogen). J2‐3T3 fibroblasts, a gift of Dr. Howard Green (Harvard Medical School), were cultured in DMEM with high glucose, 10% bovine serum, and 1% penicillin–streptomycin. Liver endothelial cell line TMNK‐1 (“LEC”), a gift from Dr. Naoya Kobayashi (Okayama University), were cultured in DMEM with high glucose, 10% FBS, and 1% penicillin‐streptomycin. Cells were used between passages 7 and 19.

Stevens K.R., Miller J.S., Blakely B.L., Chen C.S, & Bhatia S.N. (2015). Degradable hydrogels derived from PEG‐diacrylamide for hepatic tissue engineering. Journal of Biomedical Materials Research. Part a, 103(10), 3331-3338.

+ Open protocol

+ Expand

Aortic SMC Cholesterol Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols

Aortic SMCs were explanted from the ascending aortas of Perk^SMC−/− and Perk^SMC+/+ mice as described earlier.²⁶ SMCs were treated with indicated amounts of free cholesterol complexed to methyl-β-cyclodextrin (MBD-Chol, Millipore Sigma) in DMEM containing high glucose (Cellgro), 10% FBS (Gibco), 1% antibiotic/anti-mycotic (Millipore Sigma), and 0.2% BSA (Fisher Scientific) for 72 hours at 37 °C and 5% CO₂.

Chattopadhyay A., Guan (关蒲骏) P., Majumder S., Kaw K., Zhou (周桢) Z., Zhang C., Prakash S.K., Kaw A., Buja L.M., Kwartler C.S, & Milewicz D.M. (2022). Preventing Cholesterol-Induced Perk (Protein Kinase RNA-Like Endoplasmic Reticulum Kinase) Signaling in Smooth Muscle Cells Blocks Atherosclerotic Plaque Formation. Arteriosclerosis, Thrombosis, and Vascular Biology, 42(8), 1005-1022.

+ Open protocol

+ Expand

Isolation and Culture of Rat Hepatocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rat hepatocytes were isolated from 2 – 3 month old female Lewis rats (Charles River) by collagenase perfusion as described previously^{28 (link)-30 (link)}. Briefly, animals were anesthetized and the portal vein was cannulated. The liver was then perfused with buffers and digested with collagenase. The resultant digest was purified using Percoll centrifugation. Hepatocytes were cultured in DMEM with high glucose (Cellgro), 10% (v/v) fetal bovine serum (Gibco), 0.5 U/ml insulin (Lilly), 7 ng/ml glucagons (Bedford Laboratories), 7.5 μg/ml hydrocortisone (Sigma), and 1% penicillin-streptomycin (‘Hepatocyte media’, Invitrogen). J2-3T3 fibroblasts, a gift of Dr. Howard Green (Harvard Medical School), were cultured in DMEM with high glucose, 10% bovine serum, and 1% penicillin-streptomycin. Liver endothelial cell line TMNK-1 (‘LEC’), a gift from Dr. Naoya Kobayashi (Okayama University), were cultured in DMEM with high glucose, 10% FBS, and 1% penicillin-streptomycin. Cells were used between passages 7 and 19.

+ Open protocol

+ Expand

Cytotoxicity of PDA-F on HCT116 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

In vitro cytotoxicity of PDA-F was performed on HCT116 cell lines using the MTS assay. HCT116 colorectal carcinoma cells were plated at

2.5 \times 10^{3}

cells/well in DMEM (Eagle’s minimal essential medium) high glucose (Corning) + 10% FBS (fetal bovine serum). Cell were grown for 24 h and then treated with aqueous suspensions of PDA-F (c = 0 to 500 μg/mL). After incubation for 24 h, cells were washed twice with PBS (phosphate-buffered saline) and additional medium (100 μL) was added to each well, followed by CellTiter Aqueous One Solution (Promega, 20 μL). After 3 h at 37 °C, the number of viable cells was determined by comparison of absorbance readings at λ = 490 nm (test wavelength) and λ = 690 nm (reference wavelength).

Xie Y., Wang J., Wang Z., Krug K.A, & Rinehart J.D. (2018). Perfluorocarbon-loaded polydopamine nanoparticles as ultrasound contrast agents. Nanoscale, 10(26), 12813-12819.

+ Open protocol

+ Expand

Culturing Colon Cancer and Normal Colon Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Colon cancer cell lines (SW480 and SW62) were obtained due to the courtesy of Prof. Caroline Dive, Paterson Institute for Cancer Research, University of Manchester. Human colon carcinoma cell line HCT116 and fetal colon cell line FHC (CRL-1831) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and maintained according to the ATCC’s instructions. HCT116 cells were cultured in McCoy’s 5A medium (Gibco, Paisley, UK). SW480 and SW620 cells were cultured in DMEM (Dulbecco’s modified eagle medium) with high glucose (Corning, NY, USA). FHC cells were cultured in DMEM/F12 medium (Gibco) supplemented with 25 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), 10 ng/mL cholera toxin, 0.005 mg/mL insulin, 0.005 mg/mL transferrin, and 100 ng/mL hydrocortisone. All media were supplemented with 10% fetal bovine serum (FBS, Gibco) and ciprofloxacin (10 µg/mL). The cells were cultured by biweekly passages in a 37 °C humidified atmosphere with 5% CO₂ and regularly tested for Mycoplasma sp. contamination.

Klebowski B., Depciuch J., Stec M., Krzempek D., Komenda W., Baran J, & Parlinska-Wojtan M. (2020). Fancy-Shaped Gold–Platinum Nanocauliflowers for Improved Proton Irradiation Effect on Colon Cancer Cells. International Journal of Molecular Sciences, 21(24), 9610.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!