Formalin-fixed paraffin-embedded samples were processed through standard deparaffinization protocols. Antigen retrieval was performed in 90°C citrate buffer until the temperature reached 55°C. The tissue was then incubated in blocking buffer (5% normal goat serum, 2.5% bovine serum albumin [BSA] in PBS at pH 7.5) for 30 minutes. Anti-RUNX2 (clone M-70, Santa Cruz Biotechnology, Dallas, TX, USA) antibodies were incubated overnight at 4°C in a humidified chamber followed by the DAKO Envision Visualization system and counterstaining with hematoxylin.
Anti runx2 clone m 70
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-RUNX2 (clone M-70) is a primary antibody reagent that specifically recognizes the RUNX2 protein. RUNX2 is a transcription factor that plays a crucial role in osteoblast differentiation and bone formation. This antibody can be used for applications such as Western blotting, immunohistochemistry, and immunofluorescence to detect and study the RUNX2 protein.
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Lab products found in correlation
2 protocols using anti runx2 clone m 70
1
Immunohistochemical Analysis of RUNX2 Expression
2
Immunohistochemical Analysis of RUNX2
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Formalin‐fixed paraffin‐embedded samples were processed through standard deparaffinization protocols. Antigen retrieval was performed in 90°C citrate buffer until the temperature reached 55°C. The tissue was then incubated in blocking buffer (5% normal goat serum, 2.5% bovine serum albumin [BSA] in PBS at pH 7.5) for 30 minutes. Anti‐RUNX2 (clone M‐70, Santa Cruz Biotechnology, Dallas, TX, USA) antibodies were incubated overnight at 4°C in a humidified chamber followed by the DAKO Envision Visualization system and counterstaining with hematoxylin.
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