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Anti ki67 bv650

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Anti-Ki67 BV650 is a fluorochrome-conjugated antibody that binds to the Ki67 protein, which is a cellular marker for proliferation. This product can be used in flow cytometry and other immunoassay applications to detect and quantify proliferating cells.

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Lab products found in correlation

Fluorescently labelled antibodies, by BD (2 mentions) Live dead fixable blue dead cell stain kit, by Thermo Fisher Scientific (2 mentions) Facs symphony, by BD (2 mentions) Cd8 buv805 clone rpa t8, by Beckman Coulter (2 mentions) Cd20 cy5 pe clone 2h7, by Beckman Coulter (2 mentions) Nkg2a pe cy7 clone z199, by Beckman Coulter (2 mentions) Hla dr bv480 clone g46 6, by Beckman Coulter (2 mentions) Cd64 buv395 clone 10.1, by Beckman Coulter (2 mentions) Cd80 bv421, by Beckman Coulter (2 mentions) Cd169 bb515, by Beckman Coulter (2 mentions) Cd69 ecd clone tp1.55.3, by Beckman Coulter (2 mentions) Transcription factor buffer set, by BD (2 mentions)

Spelling variants of this product

Anti-Ki67 (102 citations)

2 protocols using anti ki67 bv650

1

Multiparametric Flow Cytometry Analysis of PBMCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cryopreserved PBMC were thawed, washed, then stained with LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation (Thermo Fisher) for 20 min at room temperature. Cells were stained with fluorescently labelled antibodies (all BD Biosciences unless otherwise stated) specific for cell surface markers CD3 Cy7 APC (clone SP34-2), CD4 Cy5.5 PE (clone S3.5; Life Technologies), CD8 BUV805 (clone RPA-T8), CD20 Cy5 PE (clone 2H7), NKG2A PE Cy7 (clone Z199; Beckman Coulter), CD14 BV750 (clone M5E2), CD16 BV570 (clone 3G8; BioLegend), CD28 APC (clone CD28.2), CD45RA BUV737 (clone 5H9), CD38 PE (OKT10; Capricio Biotechnologies), CD69 ECD (clone TP1.55.3; Beckman Coulter), HLA-DR BV480 (clone G46-6), CD64 BUV395 (clone 10.1), CD80 BV421 (clone L307.4) and CD169 BB515 (clone 7-239). In some experiments, cells were fixed and permeabilized using the Transcription Factor Buffer Set (BD Biosciences) according to the manufacturer’s instructions prior to staining with the anti-Ki67 BV650 (clone B56). Finally, cells were resuspended in 0.5% paraformaldehyde and staining was measured on a BD FACS Symphony. Data was analyzed using FlowJo v10 software (Tree Star, inc.).
King H.A., Pokkali S., Kim D., Brammer D., Song K., McCarthy E., Lehman C., Todd J.P., Foulds K.E., Darrah P.A., Seder R.A., Bolton D.L, & Roederer M. (2023). Immune Activation Profiles Elicited by Distinct, Repeated TLR Agonist Infusions in Rhesus Macaques. The Journal of Immunology Author Choice, 211(11), 1643-1655.
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2

Multiparametric Flow Cytometry Analysis of PBMCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cryopreserved PBMC were thawed, washed, then stained with LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation (Thermo Fisher) for 20 min at room temperature. Cells were stained with fluorescently labelled antibodies (all BD Biosciences unless otherwise stated) specific for cell surface markers CD3 Cy7 APC (clone SP34-2), CD4 Cy5.5 PE (clone S3.5; Life Technologies), CD8 BUV805 (clone RPA-T8), CD20 Cy5 PE (clone 2H7), NKG2A PE Cy7 (clone Z199; Beckman Coulter), CD14 BV750 (clone M5E2), CD16 BV570 (clone 3G8; BioLegend), CD28 APC (clone CD28.2), CD45RA BUV737 (clone 5H9), CD38 PE (OKT10; Capricio Biotechnologies), CD69 ECD (clone TP1.55.3; Beckman Coulter), HLA-DR BV480 (clone G46-6), CD64 BUV395 (clone 10.1), CD80 BV421 (clone L307.4) and CD169 BB515 (clone 7-239). In some experiments, cells were fixed and permeabilized using the Transcription Factor Buffer Set (BD Biosciences) according to the manufacturer’s instructions prior to staining with the anti-Ki67 BV650 (clone B56). Finally, cells were resuspended in 0.5% paraformaldehyde and staining was measured on a BD FACS Symphony. Data was analyzed using FlowJo v10 software (Tree Star, inc.).
King H.A., Pokkali S., Kim D., Brammer D., Song K., McCarthy E., Lehman C., Todd J.P., Foulds K.E., Darrah P.A., Seder R.A., Bolton D.L, & Roederer M. (2023). Immune Activation Profiles Elicited by Distinct, Repeated TLR Agonist Infusions in Rhesus Macaques. The Journal of Immunology Author Choice, 211(11), 1643-1655.
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