Cells treated as described
above were resuspended in 150 μL of PBS after each experiment
and dropped onto an uncoated microslide with coverslip (MatTek Corp.).
Confocal fluorescence microscopy (CFM) experiments were performed
using a Zeiss LSM 510 META NLO two-photon laser scanning confocal
microscope system, operating at a 488 nm excitation wavelength and
at 527 ± 23 nm detecting emission wavelength using a 505–550
nm bandpass filter. Images were captured using a C-Apochromat 63x/1.2 water (corr) objective. Images for conjugate2 (Figure 6 ) were obtained using the
camera mode with a filter set of 350 ± 25 nm excitation wavelength
and 420 nm long pass emission wavelength. Acquired data were analyzed
using LSM 510 Meta software.
above were resuspended in 150 μL of PBS after each experiment
and dropped onto an uncoated microslide with coverslip (MatTek Corp.).
Confocal fluorescence microscopy (CFM) experiments were performed
using a Zeiss LSM 510 META NLO two-photon laser scanning confocal
microscope system, operating at a 488 nm excitation wavelength and
at 527 ± 23 nm detecting emission wavelength using a 505–550
nm bandpass filter. Images were captured using a C-Apochromat 63x/1.2 water (corr) objective. Images for conjugate
camera mode with a filter set of 350 ± 25 nm excitation wavelength
and 420 nm long pass emission wavelength. Acquired data were analyzed
using LSM 510 Meta software.