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κ fluorescein isothiocyanate and λ pe immunoglobulin light chains

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κ-fluorescein isothiocyanate and λ-PE immunoglobulin light chains are lab equipment used for analytical purposes. They are fluorescent dyes that can be used to label and detect specific proteins or cells in a sample. The κ-fluorescein isothiocyanate emits green fluorescence, while the λ-PE emits red fluorescence when excited by the appropriate wavelength of light.

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Lab products found in correlation

Percp cy5, by BD (2 mentions) Fcs express, by De Novo Software (2 mentions) Cd20 apc cy7, by BD (2 mentions) Facscanto flow cytometer, by BD (2 mentions) Cd45 phycoerythrin pe cy7, by BD (2 mentions)

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Immunoglobulin κ and λ light chains (2 citations)

2 protocols using κ fluorescein isothiocyanate and λ pe immunoglobulin light chains

1

Six-Color Flow Cytometry for B-Cell Clonality

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Six color flow cytometry was performed using CD45 and side scatter gating. While the complete panel of antibodies employed varied depending on the available number of cells, B-cell clonality was evaluated in all cases using the following six color combination: CD5-perdinin chlorophyll protein complex-Cy5.5 (PerCP-Cy5.5), CD19-allophycocyanin (APC), CD20-APC-Cy7, CD45-phycoerythrin (PE)-Cy7, and κ-fluorescein isothiocyanate and λ-PE immunoglobulin light chains (Becton Dickenson, San Jose, CA). Analysis was completed using an FACSCanto flow cytometer (Becton Dickinson). Flow cytometry data were analyzed using FCS Express (v3.0, De Novo Software, Los Angeles, CA).
Guo L., Wang Z., Anderson C.M., Doolittle E., Kernag S., Cotta C.V., Ondrejka S.L., Ma X.J, & Cook J.R. (2017). Ultrasensitive Automated RNA in situ Hybridization for Kappa and Lambda Light Chain mRNA Detects B-cell Clonality in Tissue Biopsies with Performance Comparable or Superior to Flow Cytometry. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 31(3), 385-394.
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2

Six-Color Flow Cytometry for B-Cell Clonality

Check if the same lab product or an alternative is used in the 5 most similar protocols
Six color flow cytometry was performed using CD45 and side scatter gating. While the complete panel of antibodies employed varied depending on the available number of cells, B-cell clonality was evaluated in all cases using the following six color combination: CD5-perdinin chlorophyll protein complex-Cy5.5 (PerCP-Cy5.5), CD19-allophycocyanin (APC), CD20-APC-Cy7, CD45-phycoerythrin (PE)-Cy7, and κ-fluorescein isothiocyanate and λ-PE immunoglobulin light chains (Becton Dickenson, San Jose, CA). Analysis was completed using an FACSCanto flow cytometer (Becton Dickinson). Flow cytometry data were analyzed using FCS Express (v3.0, De Novo Software, Los Angeles, CA).
Guo L., Wang Z., Anderson C.M., Doolittle E., Kernag S., Cotta C.V., Ondrejka S.L., Ma X.J, & Cook J.R. (2017). Ultrasensitive Automated RNA in situ Hybridization for Kappa and Lambda Light Chain mRNA Detects B-cell Clonality in Tissue Biopsies with Performance Comparable or Superior to Flow Cytometry. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 31(3), 385-394.
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