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H 2 dk 15 5 5

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The H-2 Dk (15-5-5) is a multi-parameter flow cytometry reagent that contains fluorochrome-conjugated antibodies targeting the H-2Dk mouse major histocompatibility complex (MHC) class I molecule. This reagent is designed for the identification and analysis of H-2Dk-expressing cells in mouse samples.

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Lab products found in correlation

70 μm cell strainer, by Thermo Fisher Scientific (2 mentions) H 2 db kh95, by BD (2 mentions) H 2kk 36 7 5, by BioLegend (2 mentions) Facscalibur, by BD (2 mentions)

2 protocols using h 2 dk 15 5 5

1

Murine MHC Typing by Flow Cytometry

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MHC typing was performed by flow cytometry. Spleens were pressed through a 70 μm cell strainer (Thermo Fisher Scientific, Ottawa, Ontario, Canada). Spleen cell suspensions were treated with NH4Cl to lyse red blood cells. Single cell suspensions were stained with the following antibodies: I-Ak (10-3.6), I-Ak (11-5.2), I-Ab (AF6-120.1), I-E (14.4.4S), H-2Kk (36-7-5), H-2 Dk (15-5-5)(Biolegend, San Diego, CA, USA) and H-2 Db (KH95; BD Biosciences, Mississauga, Ontario, Canada), Flow cytometry data were acquired on an FACSCalibur instrument (BD Biosciences) and analyzed with FlowJo software (TreeStar, Ashland, OR, USA).
Pelletier A.N., Aliesky H.A., Banuelos B., Chabot-Roy G., Rapoport B., Lesage S, & McLachlan S.M. (2015). Evidence that MHC I-E dampens thyroid autoantibodies and prevents spreading to a second thyroid autoantigen in I-Ak NOD mice. Genes and immunity, 16(4), 268-274.
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2

Murine MHC Typing by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
MHC typing was performed by flow cytometry. Spleens were pressed through a 70 μm cell strainer (Thermo Fisher Scientific, Ottawa, Ontario, Canada). Spleen cell suspensions were treated with NH4Cl to lyse red blood cells. Single cell suspensions were stained with the following antibodies: I-Ak (10-3.6), I-Ak (11-5.2), I-Ab (AF6-120.1), I-E (14.4.4S), H-2Kk (36-7-5), H-2 Dk (15-5-5)(Biolegend, San Diego, CA, USA) and H-2 Db (KH95; BD Biosciences, Mississauga, Ontario, Canada), Flow cytometry data were acquired on an FACSCalibur instrument (BD Biosciences) and analyzed with FlowJo software (TreeStar, Ashland, OR, USA).
Pelletier A.N., Aliesky H.A., Banuelos B., Chabot-Roy G., Rapoport B., Lesage S, & McLachlan S.M. (2015). Evidence that MHC I-E dampens thyroid autoantibodies and prevents spreading to a second thyroid autoantigen in I-Ak NOD mice. Genes and immunity, 16(4), 268-274.
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