Horseradish peroxidase linked anti mouse immunoglobulin g secondary antibody 7076s

BPA, BPB, BPS, BPF, BPAF, AO44S25, 4-OP, 4-NP, 4OH-TAM, sodium nitrite-¹⁵N, and SYPRO Orange dye were purchased from Sigma-Aldrich (Oakville, ON, Canada). HisPur Ni-NTA (nitrilotriacetic acid) magnetic beads and Zeba 7K spin desalting plates were purchased from Thermo Fisher Scientific (Whitby, ON, Canada). Human ERRγ open reading frame (ORF) clone (BC064700.1) and pET28-MHL vector were provided by Structural Genomics Consortium. BL21(DE3) competent E. coli was obtained from BioLabs Inc. (Whitby, ON, Canada). Antibody to histidine was purchased from Bioshop (Burlington, ON, Canada; TAG001.100) and was diluted with 5% skim milk at a dilution of 1:1000 for Western blotting. Horseradish peroxidase–linked anti-mouse immunoglobulin G secondary antibody (7076S) was purchased from Cell Signaling Technology (Beverly, Massachusetts, USA), which was used at a dilution of 1:20,000 in 5% skim milk.

BPA, BPB, BPS, BPF, BPAF, AO44S25, 4-OP, 4-NP, 4OH-TAM, sodium nitrite-¹⁵N, and SYPRO Orange dye were purchased from Sigma-Aldrich (Oakville, ON, Canada). HisPur Ni-NTA (nitrilotriacetic acid) magnetic beads and Zeba 7K spin desalting plates were purchased from Thermo Fisher Scientific (Whitby, ON, Canada). Human ERRγ open reading frame (ORF) clone (BC064700.1) and pET28-MHL vector were provided by Structural Genomics Consortium. BL21(DE3) competent E. coli was obtained from BioLabs Inc. (Whitby, ON, Canada). Antibody to histidine was purchased from Bioshop (Burlington, ON, Canada; TAG001.100) and was diluted with 5% skim milk at a dilution of 1:1000 for Western blotting. Horseradish peroxidase–linked anti-mouse immunoglobulin G secondary antibody (7076S) was purchased from Cell Signaling Technology (Beverly, Massachusetts, USA), which was used at a dilution of 1:20,000 in 5% skim milk.