Acetonitrile mecn

Optical rotations were obtained using a Horiba SEPA-300 instrument (Horiba, Kyoto, Japan). IR spectra were recorded on a JASCO FT/IR 4100 spectrometer using an NaCl cell. ¹H and ¹³C NMR spectra were recorded using a JNM-EX 400 (400 MHz and 100 MHz) spectrometer (see Supplementary Materials). N,N-dimethylformamide (DMF), methanol (MeOH), and acetonitrile (MeCN) were purchased from Kanto Chemical Co. Inc. (Tokyo, Japan). Dichloromethane (CH₂Cl₂) was distilled from CaH₂. All commercially available reagents were employed as received. Analytical TLC was carried out using pre-coated silica gel plates (TLC silica gel 60 F₂₅₄). The silica gel used for column chromatography was Wakogel 60N 63–212 μm.

The Ir(III) complexes used in this study were synthesized and characterized according to the methods described in the Supplementary Information.
Acetonitrile (MeCN; Kanto Chemical, spectroscopic grade) and NH₄Cl (Kanto Chemical, special grade) were used as received. Antimycin A from Streptomyces sp. (Ant A) and carbonyl cyanide 4-(trifluoromethoxy) phenylhydrozone (FCCP, 98%) were purchased from Sigma-Aldrich. Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Laboratories.
¹H-NMR spectra were recorded with a JEOL JNM-ECS400 (400 MHz) spectrometer in DMSO-d₆. ¹H-NMR chemical shifts were referenced to tetramethylsilane. The apparent resonance multiplicity was described as s (singlet), d (doublet), dd (double doublet), t (triplet), and m (multiplet). ESI–MS spectra were recorded on an Applied Biosystems API 2000 mass spectrometer.