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2 protocols using paclobutrazole

1

Comprehensive Analysis of Fungicide Residues

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Seventeen agricultural fungicides (test range 32–0.03 mg/L), five azole fungicide metabolites (test range 16–0.016 mg/L) and four medical azoles were included (test range 32–0.03 mg/L). The agricultural fungicides (bixafen, boscalid, fluxapyroxad, fluopyram, folpet, azoxystrobin, prothioconazole, paclobutrazole, epoxiconazole, propiconazole, tebuconazole, difenoconazole, metconazole, prothiozonacole-desthio, prochloraz, imazalil were purchased from Sigma-Aldrich, Søborg, Denmark, whereas mefentrifluconazol was purchased from LGC Standards (Teddington, Middlesex, United Kingdom www.lgcstandards.com, accessed on 26 January 2021). The metabolites included: 1,2,3-triazole (Sigma-Aldrich, Søborg, Denmark), 1,2,4-triazole and triazole sulfonamide (Toronto Research Chemicals, Toronto, Canada), and triazole alanine and triazole acetate (HPC Standards GmbH, Borsdorf, Germany). The medical antifungal agents used were itraconazole, voriconazole, isavuconazole and posaconazole (all from Sigma-Aldrich, Søborg, Denmark, except isavuconazole, which derived from Basilea).
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2

Optimizing Humic Acid, Boron, and Paclobutrazole for Crop Yield

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The humic acid (obtained from Agrolink Agricultural Co., Roxy, Cairo, Egypt) was added to the irrigation water at a concentration of 2 gL -1 . Boron (B) (used in the form of boric acid;170 g Bkg - 1 ) as well as Paclobutrazole (PP333) was obtained from Sigma Aldrich .Thus there were 18 treatment combinations (2 H X 3 B X 3 PP333) .Treatments were in 3 replicates . After two months from replanting, the plants were fertilized every month with NPK fertilizer using ammonium sulfate (20.5% N), calcium superphosphate (15.5% P2O5) and potassium sulfate (48% K2O). A mixture of the three fertilizers, with a ratio of 1: 1: 1 (N: P2O5: K2O), was prepared and applied to the pots at the rate of 5 g/pot. Common agricultural practices (irrigation, manual weed control, . . . etc.) were carried out when needed.
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