HCC specimens were collected during surgery. The proteins separated on gels after electrophoresis are transferred to PVDF membranes and subsequently incubated with primary and secondary antibodies. The following primary antibodies were incubated for 12 h at 4 °C: GAPDH (1:10,000, ABclonal), YTHDF1 (1:1000, ABclonal), YTHDF2 (1:1000, ABclonal) and YTHDF3 (1:1000, ABclonal), KIAA1429(1:1000, Proteintech), METTL3(1:1000, Proteintech), RBM15(1:1000, Proteintech), ZC3H13(1:1000, Proteintech), METTL16(1:1000, Proteintech), ALKBH5(1:1000, Proteintech), METTL14(1:1000, Proteintech), WTAP(1:1000, Proteintech), HNRNPC(1:1000, Proteintech), YTHDC1(1:1000, Proteintech), YTHDC2(1:1000, Proteintech), FTO(1:1000, Proteintech). The secondary antibody (1:5000, ABclonal) was incubated for 2 h at room temperature. Immunoreactive bands were developed using an enhanced chemiluminescence detection kit (Genview Scientific Inc., USA). All experiments were performed in triplicate.
Ythdf1
Manufactured by ABclonal
Sourced in China
YTHDF1 is a lab equipment product manufactured by ABclonal. It is a tool for scientific research and experimentation. The core function of YTHDF1 is to facilitate the study of RNA-binding proteins and their interactions with RNA molecules.
Automatically generated - may contain errors
Lab products found in correlation
Alkbh5, by Proteintech (1 mentions)
Ythdc1, by Proteintech (1 mentions)
Kiaa1429, by Proteintech (1 mentions)
Mettl14, by Proteintech (1 mentions)
Rbm15, by Proteintech (1 mentions)
Hnrnpc, by Proteintech (1 mentions)
Mettl16, by Proteintech (1 mentions)
Gapdh, by ABclonal (1 mentions)
Mettl3, by Proteintech (1 mentions)
Mettl14, by ZenBio (1 mentions)
Ythdf3, by ABclonal (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
β actin, by Abcam (1 mentions)
Ripa lysis buffer, by Thermo Fisher Scientific (1 mentions)
2 protocols using ythdf1
1
Western Blot Analysis of m6A Regulators in HCC
2
Profiling m6A Regulators in Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
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For western blot, 100mg of fresh tissues were isolated, homogenized and added into RIPA Lysis Buffer (Thermo Scienti c, USA). After ultrasonic treatment, the proteins of the lysated samples were prepared.
Protein was quanti ed and separated by SDS-PAGE, then electrically transferred to a polyvinylidene uoride (PVDF) membrane (Millipore, USA). The primary antibodies of METTL3 (Zen bioscience, China), METTL14 (Zen bioscience, China), FTO (Zen bioscience, China), YTHDF1 (ABclonal, China), YTHDF3 (ABclonal, China) and β-actin (Abcam, UK) were diluted in proportion of 1:100 and incubated with the PVDF membrane, then incubated with the secondary antibodies labeled by HRP (Abcam, UK). ECL chemiluminescence method was used for testing.
Protein was quanti ed and separated by SDS-PAGE, then electrically transferred to a polyvinylidene uoride (PVDF) membrane (Millipore, USA). The primary antibodies of METTL3 (Zen bioscience, China), METTL14 (Zen bioscience, China), FTO (Zen bioscience, China), YTHDF1 (ABclonal, China), YTHDF3 (ABclonal, China) and β-actin (Abcam, UK) were diluted in proportion of 1:100 and incubated with the PVDF membrane, then incubated with the secondary antibodies labeled by HRP (Abcam, UK). ECL chemiluminescence method was used for testing.
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