Cell culture reagents (RPMI-1640 medium without glucose and fetal bovine serum) were purchased from Gibco, California, USA. The [9,10-3H]-palmitic acid, [3H]-H2O, 2-[1,2-3H]-deoxy-D-glucose, [1-14C]-mannitol, and liquid scintillation counting cocktail were from Perkin Elmer, Massachusetts, USA. Hydrocortisone sodium phosphate, as GC treatment used in this study, was purchased from Nycomed Pharma (Zurich, Switzerland). Lipoprotein lipase (LPL) was purchased from Sigma-Aldrich (St. Louis, USA). MTT for the colorimetric assay for the nonradioactive quantification of cellular metabolic activity was purchased from Roche (Mannheim, Germany). Apoptosis in the placental explants was analyzed by the Dead-End TM Fluorometric TUNEL System Kit (Promega, Madison, WI, USA) and the Caspase-GLO 3/7 Assay Kit (Promega, Madison, USA).
Liquid scintillation counting cocktail
Manufactured by PerkinElmer
Sourced in United States
Liquid scintillation counting cocktail is a solution designed to be used in liquid scintillation counters. It enables the detection and quantification of radioactive samples by converting the energy from radioactive decay into light that can be measured by the instrument.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 medium without glucose, by Thermo Fisher Scientific (2 mentions)
Deadend fluorometric tunel system kit, by Promega (1 mentions)
3h h2o, by PerkinElmer (1 mentions)
9 10 3h palmitic acid, by PerkinElmer (1 mentions)
Caspase glo 3 7 assay kit, by Promega (1 mentions)
2 deoxy d 1 2 3h glucose, by PerkinElmer (1 mentions)
Methotrexate, by Merck Group (1 mentions)
Wortmannin, by Merck Group (1 mentions)
Recombinant human hgf, by Merck Group (1 mentions)
2 deoxy glucose 2 dog, by Merck Group (1 mentions)
Cytochalasin b, by Merck Group (1 mentions)
2 protocols using liquid scintillation counting cocktail
1
Cell Culture and Radiotracer Assays for Placental Metabolism
2
Radioactive Tracer Uptake in Cell Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cell culture reagents (RPMI-1640 medium without glucose) and fetal bovine serum were from Invitrogen/Gibco, California, USA. The [9,10-3 [1,2-14 C]-acetic acid, L-[N-methyl- 14 C]-carnitine-HCl and liquid scintillation counting cocktail were from PerkinElmer, Massachusetts, USA. Methotrexate, Wortmannin, 2-deoxy-glucose (2-DOG) and cytochalasin B were purchased from Sigma-Aldrich (Madrid, Spain). Recombinant human HGF was purchased from Millipore Iberica (Madrid, Spain).
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