The following antibodies were used for phenotyping and proliferation assays: anti-CD4 BUV496 (clone GK1.5, BD), anti-CD25 PE (clone PC61, Biolegend), anti-CD62L APC (clone MEL-14, BD), anti-CD69 PE-Cy7 (clone H1.2F3, BD), anti-Foxp3 AF488 (clone FJK-16s, Invitrogen), anti-myc tag AF488/AF647 (clone 9B11, CellSignaling), anti-EGFRt PE/BV711 (clone AY13, Biolegend), anti-Thy1.1 PE-Cy7 (clone HIS51, eBioscience), anti-CD45.1 PE-Cy7 (clone A20, Biolegend), CellTrace Violet (CTV, ThermoFisher), Fixable Viability Dye eFluor780 (ThermoFisher).
Anti cd25 pe clone pc61
Manufactured by BioLegend
Anti-CD25 PE (clone PC61) is a fluorescently labeled antibody that specifically binds to the CD25 surface marker. CD25 is the alpha chain of the interleukin-2 receptor, which is expressed on activated T cells, regulatory T cells, and other immune cell types. This product can be used for the identification and characterization of CD25-expressing cells in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Celltrace violet ctv, by Thermo Fisher Scientific (1 mentions)
Fixable viability dye efluor 780, by Thermo Fisher Scientific (1 mentions)
Anti cd62l apc clone mel 14, by BD (1 mentions)
Facscanto 2, by BD (1 mentions)
Anti cd11b bv421 clone m1 70, by BioLegend (1 mentions)
Anti f4 80 pe bm8, by BioLegend (1 mentions)
Anti cd11b fitc clone m1 70, by BioLegend (1 mentions)
Anti cd27 apc clone l g 3a10, by BioLegend (1 mentions)
Anti cd8 fitc clone 53.6.7, by BioLegend (1 mentions)
Anti cd3 bv421 clone 17a2, by BioLegend (1 mentions)
Fortessa, by BD (1 mentions)
Apc cy7 anti cd45 30 f11, by BioLegend (1 mentions)
2 protocols using anti cd25 pe clone pc61
1
Multifaceted Immune Cell Analysis
2
Multiparameter Flow Cytometry Analysis
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For FACS analysis, the following antibodies were used: anti-CD3-Bv421-(clone 17A2, Biolegend), anti-CD4-Bv421 (clone RM 4-5, eBioscience), anti-CD8-FITC (clone 53-6.7, Biolegend), anti-CD25 PE (clone PC61, Biolegend), anti-CD27-APC (clone LG.3A10, Biolegend), anti-CD11b-FITC (clone M1/70, Biolegend), anti-CD11b-Bv421 (clone M1/70, Biolegend), anti-FceRIα-PE (MAR-1, Biolegend), anti-F4/80-PE (BM8, Biolegend), anti-CD45-APC/Cy7 (30-F11, Biolegend), anti-CD107a-FITC (1D4B, Biolegend) and anti-P2X7-AF647 (clone Hano44, UKE) (Adriouch et al., 2005) (link). Flow cytometric analyses were performed on a BD Fortessa (Beckton Dickinson) or a BD FACS CantoII (Beckton Dickinson).
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