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4 protocols using bis trimethylsilyl trifluoroacetamide bstfa 1 trimethylchlorosilane tmcs

1

Cholesterol Oxidation Compound Analysis

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Cholesterol, linoleic acid, oleic acid, Cholesterol oxide standards (7-keto, 6-keto, 7α-OH, 7β-OH, 5,6α-EP, 5,6β-EP, 25-OH, 20-OH, and triol), butylated hydroxytoluene (BHT), pyridine, and silicic acid (100 mesh) were purchased from Sigma-Aldrich Co., LLC (Seoul, Korea). Bis-[trimethylsilyl]-trifluoroacetamide (BSTFA) + 1 % trimethylchlorosilane (TMCS) was obtained from Supelco (Bellefonte, PA, USA). HPLC grade hexane, ethyl acetate, acetone, methanol, and chloroform, Celite 545, and calcium phosphate (CaHPO4.2H2O) were purchased from Fisher Scientific Co. (Malvern, PA, USA).
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2

Cholesterol Oxidation Products Analysis

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Cholesterol, COPs standards [7-ketocholesterol (7-keto), 6-ketocholesterol (6-keto), 7α-hydroxy-cholesterol (7α-OH), 7β-hydroxycholesterol (7β-OH), 5,6α-epoxycholesterol (5,6α-EP), 5,6β-epoxycholesterol (5,6β-EP), 25-hydroxycholesterol (25-OH), 20-hydroxycholesterol (20-OH), and cholestanetriol (triol)], butylated hydroxytoluene (BHT), pyridine, and silicic acid (100 mesh) were purchased from Sigma-Aldrich Co., LLC (Korea). Bis-(trimethylsilyl)-trifluoroacetamide (BSTFA) + 1% trimethylchlorosilane (TMCS) was obtained from Supelco (Bellefonte, USA). HPLC grade hexane, ethyl acetate, acetone, methanol, and chloroform, Celite 545, and calcium phosphate (CaHPO4.2H2O) were purchased from Fisher Scientific Co. (USA).
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3

Cholesterol Oxidation Product Analysis

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Cholesterol, linoleic acid, oleic acid, COPs standards [7-ketocholesterol
(7-keto), 6-ketocholesterol (6-keto), 7α-hydroxy-cholesterol
(7α-OH), 7β-hydroxycholesterol (7β-OH),
5,6α-epoxycholesterol (5,6α-EP), 5,6β-epoxycholesterol
(5,6β-EP), 25-hydroxycholesterol (25-OH), 20-hydroxycholesterol (20-OH),
and cholestanetriol (triol)], butylated hydroxytoluene (BHT), pyridine, and
silicic acid (100 mesh) were purchased from Sigma-Aldrich Co., LLC (Korea).
Bis-[trimethylsilyl]-trifluoroacetamide (BSTFA)+1%
trimethylchlorosilane (TMCS) was obtained from Supelco (USA). Hexane, ethyl
acetate, acetone, methanol and chloroform of HPLC grades, celite545, and calcium
phosphate (CaHPO4·2H2O) were purchased from Fisher
Scientific Co. (USA).
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4

Lipid Profiling and Oxidation Analysis

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Cholesterol, linoleic acid, oleic acid, cholesterol oxide standards [7-ketocholesterol (7-keto), 6-ketocholesterol (6-keto), 7α-hydroxycholesterol (7α-OH), 7β-hydroxycholesterol (7β-OH), 5,6α-epoxycholesterol (5,6α-EP), 5,6β-epoxycholesterol (5,6β-EP), 25-hydroxycholesterol (25-OH), 20-hydroxycholesterol (20-OH), and cholestanetriol (triol)], butylated hydroxytoluene (BHT), Xpyridine, and silicic acid (100 mesh) were purchased from Sigma-Aldrich Co., LLC (Seoul, Korea). Bis(trimethylsilyl)trifluoroacetamide (BSTFA) + 1 % trimethylchlorosilane (TMCS) was obtained from Supelco (Bellefonte, PA, USA). HPLC-grade hexane, ethyl acetate, acetone, methanol, chloroform, Celite 545, and calcium phosphate (CaHPO4.2H2O) were purchased from Fisher Scientific Co. (Malvern, PA, USA).
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