MK-8776 (chk1 inhibitor) and BML-277 (chk2 inhibitor) were purchased from ApexBio (Houston, TX, USA). KU-60019 (ATM inhibitor) was sourced from LC-Laboratories (Woburn, MA, USA), and VE-821 (ATR inhibitor) was bought from Sellekchem (Houston, TX, USA). Inhibitors were dissolved in DMSO per the manufacturer’s direction to prepare 1000× concentrated stocks. Inhibitor concentrations were selected based on published reports in various cancer cells lines (that do not contain HPVs) and on their low toxicity in submerged PHK cultures (Figure 7 A, and data not shown).
Ku 60019
Manufactured by LC Laboratories
Sourced in United States, Germany
The KU-60019 is a laboratory equipment product designed for precise temperature measurement. It features a digital display and offers accurate temperature readings within a specified range.
Automatically generated - may contain errors
Lab products found in correlation
Mk 8776, by Apexbio (1 mentions)
Ve 821, by Selleck Chemicals (1 mentions)
Ifn γ, by Merck Group (1 mentions)
5 fluorouracil 5 fu, by Merck Group (1 mentions)
Oxaliplatin, by Merck Group (1 mentions)
Taxol, by Merck Group (1 mentions)
Flow cytometry staining buffer 1x, by R&D Systems (1 mentions)
Ve 822, by Selleck Chemicals (1 mentions)
Erlotinib, by LC Laboratories (1 mentions)
Irinotecan, by LC Laboratories (1 mentions)
Annexin 5 fitc, by BD (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Tariquidar, by MedChemExpress (1 mentions)
Calcein am, by Calibre Scientific (1 mentions)
3 protocols using ku 60019
1
Checkpoint Kinase Inhibitor Protocol
2
Drug Concentration Optimization for Cell Studies
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Stock concentrations of the compounds were prepared in Dimethyl sulfoxide (DMSO) and stored at −20°C. SM-7368 (481411-5mg), CPT (C9911-250MG), KU60019 (SML1416-5MG), irinotecan (IRI) (SKU-I1406-50MG), and VE-822 (1232416-25-9) were purchased from Sigma-Aldrich (St. Louis, MO). AZD7762 (Enzo, ENZ-CHM185-0005) from ENZO. Flow Cytometry Staining Buffer (1X) (Cat# FC001), Mouse PE-IgG2a (R&D IC003p), and Human HLA Class I (MHC I) Phycoerythrin mAb (Clone W6/32) were purchased from R&D Systems. Unless indicated otherwise, the drugs were used at the following concentrations: CPT 0.5-1 µM; SM-7368 5 µM; irinotecan 25 µg/ml; VP-16 (Etoposide, LC laboratories) 25 µM; γ-IFN (Sigma) 50 ng/ml; VE822 (Selleckchem) 100 nM; AZD7762 100 nM, KU60019 at 1 µM; erlotinib (LC laboratories) 20 µM; oxaliplatin (Sigma) 10 µM; taxol (Sigma) 50 nM; 5-Fluorouracil (5-FU, Sigma) 10 µM. For experiments in this study, single drug concentrations were pre-determined based on the length of treatment (24–48 h) that induced the expression of target proteins without visibly killing the treated cells.
3
Cytotoxicity Assay with Cell Viability Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was purchased from Sigma (Taufkirchen, Germany), annexin-V-FITC from BD Bioscience (San Jose, CA, USA); tariquidar (p-glycoprotein inhibitor) from MedChemExpress (Monmouth Junction, NJ, USA); calcein-AM from BIOZOL (Eching, Germany); VE-822 (ATR inhibitor), LY2603618 (Chk1 inhibitor), KU-60019 (ATM inhibitor), and PV1019 (Chk2 inhibitor) from LC Laboratories (Woburn, MA, USA); Anisomycin—from NeoCorp (Weilheim, Germany). Docetaxel, and doxorubicin—from a Pharmacy of the University Hospital Hamburg-Eppendorf (Hamburg, Germany). Primary and secondary antibodies used are listed in Supplementary Table S1 .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!