Vhx 6000 digital microscope

Pear peels at the equator zone and on the sunny side were sampled with a sterile blade. The peel sample was placed on a slide and viewed under a KEYENCE-VHX-6000 digital microscope in a 100-fold visual field (KEYENCE-VHX-6000, Shanghai, China) to determine fruit dot size, density, and area. The fruit dots were photographed with a microscope, and the fruit dot size was determined using plane measurement in VHXMENU. Forty fruit dots were measured for each pear. The fruit dot density was calculated by counting the number of fruit dots per unit area, as described previously [2 ]. Twenty fields of view were measured for each pear. The fruit dot area was represented by the sum of the fruit dot areas within the fruit surface unit. The 3D model of fruit dots was synthesized with the 3D synthesis option in the digital microscope system with a 500-fold view using a KEYENCE-VHX-6000 digital microscope, and the fruit dot height was determined. The fruit surface was set as a horizontal line; if the fruit dot protruded from the fruit surface, then it was marked as positive, and if the fruit dot sank into the fruit surface, it was marked as negative. Ten fruit points were measured for each pear fruit. Three biological replicates were performed, and each index was determined for nine fruits.

A latest-generation KEYENCE VHX 6000 digital microscope (Mechelen, Belgium), with an exceptional depth of focus and the ability to perform measurements and analyze living and nonliving materials, was used. This microscope had high-quality digital image recording capabilities provided by components such as the VH-Z20T (Osaka, Japan) ultra-small, high-performance zoom lens (20–200× magnification) and the VH-Z250T dual-light, high-magnification zoom lens (250–2500× magnification). The glue lines formed in the elephant dung particleboard layered with Belangke bamboo and the adhesive distribution of the core layer were analyzed using the KEYENCE VHX 6000 digital microscope at 200× magnification. In addition, a Quattro S field-emission scanning electron microscope (FE-SEM, Thermo Scientific, Vlastimila Pecha, Czech Republic.) was utilized for the analysis of references.

Directly investigated specimens were documented using either a Keyence VHX-6000 digital microscope or a super-macro-photography set-up. The latter included a Canon EOS 650D, with a Canon MP-E 65 mm macrolens. Lighting was provided by either a twin-macro flash (Yongnuo YN24EX E-TTL) or a set of two separate flashes (Yongnuo Digital Speedlite YN560EX II). Flashes and lenses were equipped with polarizers to achieve cross-polarized light.
Extant specimens were documented in 70% ethanol (original storage liquid) or under dry conditions, for specimens mounted on needles. Amber specimens were immersed with glycerol or water and a cover slip was put on top. Specimens documented on the VHX microscope were documented with ring light and cross-polarized coaxial light in front of a black and white background. The images providing the best details were further used (for details, see [88 (link)]).
All images were recorded as composite images [89 (link)], and images on the VHX microscope were additionally recorded as HDR [88 (link)]. Further processing was performed in Adobe Photoshop CS2.