The in vitro drug release behaviors of the DNVs were analyzed using the modified dialysis method shown in Figure 1 , as previously described [25 (link)]. Briefly, 1 mL IPC-DNVs or IPC-DNV gel (pre-melted at 37 °C) was transferred to a dialysis bag (diameter: 16 mm, molecular weight cut-off: 100 KD; Spectrum Chemical, New Brunswick, NJ, USA), and the bag was gently pressed to expel air and closed tightly at both ends to maintain a consistent surface area for each bag. The dialysis bag was placed in a centrifuge tube with 15 mL PBS (pH 7.4) and shaken in a constant temperature shaker at 37 °C and 80 rpm. At 1, 3, 8, and 12 h, 15 mL aliquots of release medium were removed and replaced with 15 mL of fresh, preheated medium. Each experiment was conducted in triplicate. The concentration of insulin was determined using reverse-phase, high-performance liquid chromatography with an Agilent 1200 series HPLC system (Santa Clara, CA, USA) and a 300SB-C18 column (4.6 mm × 250 mm, 5 μm.).
Dialysis bag
Manufactured by Spectrum Chemical
Sourced in United States
Dialysis bags are semi-permeable membrane devices used for the separation and purification of molecules based on their size and diffusion rate. These bags allow the selective passage of small molecules while retaining larger molecules, making them useful for various applications in research and analytical laboratories.
Automatically generated - may contain errors
Lab products found in correlation
300sb c18 column, by Agilent Technologies (1 mentions)
Agilent 1200 series hplc system, by Agilent Technologies (1 mentions)
Methacrylic anhydride, by Merck Group (1 mentions)
2 hydroxy 40 2 hydroxyethoxy 2 methylpropiophenone, by Merck Group (1 mentions)
G1890, by Merck Group (1 mentions)
1 1 diphenyl 2 picrylhydrazyl, by Merck Group (1 mentions)
Hplc grade acetonitrile, by Thermo Fisher Scientific (1 mentions)
Sodium tetraborate, by Merck Group (1 mentions)
2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid, by Merck Group (1 mentions)
Trifluoroacetic acid tfa, by Sinopharm (1 mentions)
M hydroxybiphenyl, by Merck Group (1 mentions)
1 phenyl 3 methyl 5 pyrazolone pmp, by Sinopharm (1 mentions)
4 protocols using dialysis bag
1
In Vitro Drug Release Evaluation
2
Gelatin Methacryloyl (GelMA) Hydrogel Synthesis
3
Structural Characterization and Antioxidant Evaluation of Rhubarb Polysaccharides
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RYR was purchased from Sichuan Sino‐Dandard Pharmaceutical Co. (Mianyang, Sichuan, China). Monosaccharide standards D‐glucose (Glu), D‐mannose (Man), D‐galactose (Gal), and D‐glucosamine (GlcN) were obtained from Chengdu MUST Bio‐Technology Co., Ltd. (Chengdu, Sichuan, China). Trifluoroacetic acid (TFA) and 1‐phenyl‐3‐ methyl‐5‐pyrazolone (PMP) were purchased from the SinoPharm Chemical Reagents Co. (Shanghai, China). Sodium tetraborate and m‐hydroxybiphenyl were obtained from Sigma Aldrich (St. Louis, MO, USA). Dialysis bags (molecular cutoff of 500 Da) were obtained from Spectrum Chemical Mfg. Corp. (New Brunswick, NJ, USA). Deionized water was obtained using an Ultrapure system (Chengdu, Sichuan, China). 1,1‐Diphenyl‐2‐picryl‐hydrazyl (DPPH) and 2,2‐azino‐bis‐3‐ethylbenzothiazoline‐6‐sulfonic acid (ABTS) were obtained from Sigma‐Aldrich. Hydroxyl radical assay kits were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). HPLC‐grade acetonitrile was purchased from Fisher Chemical (Waltham, MA, USA). All other chemicals were of analytical grade.
4
Nanoencapsulated Essential Oil Release
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The release profile of nanoencapsulated essential oil (i.e., EO-loaded emulsion stabilized by CNC and/or Tween 80 as shown in Section 2.3 ) was conducted according to Hou et al. [24 (link)] with modifications. Specifically, dialysis bags with a molecular weight cutoff from 12 to14 kD (Spectrum Chemical MFG Corp, New Brunswick, NJ, USA) were filled with nanoemulsion (10 mL) and dialyzed in 250 mL of deionized (DI) water with stirring. Aliquots (2 mL) of the dialysate were taken out at time intervals (2.5 min, 5 min, 10 min, 15 min, 20 min, 30 min, 1 h, 2 h, 3 h, 4 h, 5 h, 6 h, 23 h, 48 h, 72 h, and 7 days) with 2 mL of DI water being added back after collection. The absorbance of diluted dialyzed nanoemulsion in water was measured at 243 nm using a UV-Vis spectrophotometer (Azzota Scientific, Claymont, DE, USA). Using a standard curve of known essential oil concentrations (2–10 µg/mL) in 95% ethanol, the concentration of essential oil in the nanoemulsion was determined.
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