Trypsin edta

The human prostate cancer cell lines PC-3 and LNCap were purchased from the European Collection of Authenticated Cell Cultures (ECACC, Salisbury, United Kingdom). PC-3 is a cell line established from bone metastasis of grade IV prostatic adenocarcinoma from a 62-year-old male Caucasian and cells were cultured in Ham’s F-12 K (Kaighn’s) medium (Gibco, Thermo Fisher Scientific, Germany). LNCap is a cell line isolated from metastasis at the left supraclavicular lymph node of a 50-year-old patient with a confirmed diagnosis of metastatic prostate carcinoma and cells were cultured in RPMI 1640 medium (Gibco, Thermo Fisher Scientific, Germany). All cell culture media were supplemented with 10% (v/v) fetal bovine serum (FBS, Gibco, Thermo Fisher Scientific, Dreieich, Germany), 100 U/mL penicillin, and 100 mg/mL streptomycin (Biochrom GmbH, Berlin, Germany) and all cultures were maintained at 37°C with 5% (v/v) CO₂ in humidified air. Cell culture mediums were changed every 2–3 days. For sub-cultivation and experiments, cells were routinely washed with phosphate-buffered saline (PBS) without Ca⁺, Mg⁺, and phenol red (Biozym Scientific GmbH, Germany) and detached using trypsin/EDTA (Biozym Scientific GmbH, Germany). Cells were routinely tested for mycoplasma.

Human prostate cancer cell line PC-3 was purchased from the European Collection of Authenticated Cell Cultures (ECACC, Salisbury, UK). Cells were cultured in Ham’s F-12K (Kaighn’s) Medium (F12K) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 µg/mL streptomycin at 37 °C in a humidified atmosphere under 5% CO₂ in an incubator. Cells were washed with phosphate-buffered saline (PBS, Biozym Scientific GmbH, Hessisch Oldendorf, Germany) and passaged with trypsin/EDTA (Biozym Scientific GmbH, Hessisch Oldendorf, Germany). Cells were routinely tested for mycoplasma.