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2 protocols using formoterol fumarate

1

Ovalbumin-Induced Airway Inflammation Protocol

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Ovalbumin (Serva Electrophoresis GmbH, Heidelberg, Germany) was dissolved and diluted in phosphate buffered saline (PBS; Lonza, Verviers, Belgium) for the immunization, and in 0.9% saline (Delta Select GmbH, Dreieich, Germany) for the OVA challenge. Aluminium hydroxide (ImjectAlum; ThermoScientific, Rockford, IL, USA) was dissolved and diluted in PBS. Citric acid monohydrate and pyrilamine maleate were purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany) and dissolved and diluted in 0.9% saline. Respitose (Lactose monohydrate, ML003) was from Boehringer Ingelheim (Ingelheim, Germany). Codeine phosphate hemihydrate was purchased from Merck Company (Germany). Olodaterol hydrochloride, salmeterol xinafoate, formoterol fumarate, and indacaterol maleate were synthesized at the Department of Chemical Research, Boehringer Ingelheim Pharma GmbH & Co. KG (Biberach, Germany).
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2

Mast Cell Activation Assay

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Anti-DNP IgE antibody (monoclonal anti-dinitrophenyl antibody produced in mouse, IgE isotype, clone SPE-7), Evans blue, disodium cromoglycate, Desloratadine and formamide were purchased from Sigma Aldrich (Sigma-Aldrich, Co., St. Louis, MO). DNP-BSA (DNP-Albumin Conjugate, Bovine) was from Calbiochem (EMD Chemicals, Inc., San Diego, CA). Formoterol fumarate and Fostamatinib were synthesized at the Department of Chemical Research, Boehringer Ingelheim Pharma GmbH and Co. KG (Biberach, Germany). Hydroxyethylcellulose was from Merck (Merck Schuchardt OHG, Hohenbrunn, Germany). Tween20 was purchased from Acros Organics (New Jersey, USA). Narcoren was from Merial GmbH (Halbergmoos, Germany). Isoflurane (Forene) was from Abbott (Wiesbaden, Germany). Ketamine hydrochloride (Ketavet) was purchased from Pfizer Pharmacia GmbH (Berlin, Germany). Pancuronium bromide (Pancuronium Inresa) was from Inresa Arzneimittel GmbH (Freiburg, Germany).
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