Zl 55

Normal human mesothelial cells Met-5A and MPM cell lines ZL-55, REN, and MSTO were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). MPM cell lines Mero-14, Mero-41, and Mero-95 were obtained from European Collection of Authenticated Cell Culture (ECACC, Porton Down, UK). Met-5A cells were grown in Medium 199 supplemented with 10% FBS, 3.3 nM EGF, 400 nM hydrocortisone, and 870 nM zinc-free bovine insulin (all from Gibco, Carlsbad, CA, USA). Mero-14, Mero-41, and Mero-95 cells were grown in HAMS F10; ZL-55 and MSTO cells were grown in a 1:1 mixture of DMEM and Ham’s F-12; REN cells were grown in DMEM (all from Euroclone S.p.A., Milan, Italy). All MPM cells were maintained in medium supplemented with 10–15% heat-inactivated FBS, 2mM L-glutamine, 100 U/mL penicillin, and 100 U/mL streptomycin (all from Euroclone S.p.A., Milan, Italy). Cells were kept at 37 °C in a constant humidified 5% CO₂ atmosphere.

The MM cell lines ZL55, SPC212 (obtained from the University Hospital, Zurich, Switzerland) and MSTO-211H (American Type Culture Collection; ATCC, Manassas, VA, USA) were grown in RPMI1640 medium (Gibco, Basel, Switzerland) supplemented with 10% fetal bovine serum (FBS, Gibco), 100 U/mL penicillin, and 100 µg/mL streptomycin (1% PS, Gibco). The SV40-immortalized human mesothelial cell line Met-5A (ATCC) was maintained in Dulbecco’s modified Eagle’s medium/F-12 1:1 plus GlutaMax (Gibco) supplemented with 10% FBS (fetal bovine serum), 100 U/mL penicillin, and 100 µg/mL streptomycin (1% PS, Gibco).