Ab9908

Manufactured by Merck Group

Sourced in United States

The AB9908 is a piece of laboratory equipment produced by Merck Group. It is designed for general use in research and scientific settings. The core function of the AB9908 is to perform basic laboratory tasks, but a more detailed description cannot be provided while maintaining an unbiased and factual approach.

Automatically generated - may contain errors

Lab products found in correlation

Ab9904, by Merck Group (2 mentions) Ab9906, by Merck Group (2 mentions) Tgx precast gel, by Bio-Rad (1 mentions) Turboblot system, by Bio-Rad (1 mentions) β actin, by Abcam (1 mentions) Histone h3, by Abcam (1 mentions) Ab14106, by Abcam (1 mentions) Ab46794, by Abcam (1 mentions) Ahp1117, by Bio-Rad (1 mentions) A5228, by Merck Group (1 mentions)

2 protocols using ab9908

Protein Analysis by Western Blotting

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Polyacrylamide gel electrophoresis was used to resolve proteins from cellular lysates harvested in RIPA buffer. Protein determinations (BCA) were performed to ensure equal loading of protein on a per-sample basis. QKI-5, -6 and -7 were detected using primary mouse monoclonal antibodies that target pan-QKI (1:1,000, N73/168; UC Davis/NIH NeuroMab Facility), QKI-5 (1:1,000, N195A/16; UC Davis/NIH NeuroMab Facility), QKI-6 (1:1,000, N182/17; UC Davis/NIH NeuroMab Facility) or QKI-7 (1:1,000, N183/15.1; UC Davis/NIH NeuroMab Facility), or polyclonal antibodies targeting QKI-5 (1:1,000, AB9904; Millipore, Amsterdam, The Netherlands), QKI-6 (1:2,000, AB9906; Millipore) and QKI-7 (1:2,000, AB9908; Millipore). For loading references, rabbit polyclonal antibodies were used to detect β-actin or Histone H3 (both 1:4,000; Abcam, Cambridge, UK). All gels were run and blotted with Bio-Rad TGX pre-cast gels and blotted on nitrocellulose 0.2 μm using the Bio-Rad TurboBlot system (Bio-Rad Laboratories). Full blots are shown in Supplementary Fig. 7.

de Bruin R.G., Shiue L., Prins J., de Boer H.C., Singh A., Fagg W.S., van Gils J.M., Duijs J.M., Katzman S., Kraaijeveld A.O., Böhringer S., Leung W.Y., Kielbasa S.M., Donahue J.P., van der Zande P.H., Sijbom R., van Alem C.M., Bot I., van Kooten C., Jukema J.W., Van Esch H., Rabelink T.J., Kazan H., Biessen E.A., Ares Jr. M., van Zonneveld A.J, & van der Veer E.P. (2016). Quaking promotes monocyte differentiation into pro-atherogenic macrophages by controlling pre-mRNA splicing and gene expression. Nature Communications, 7, 10846.

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Antibody Characterization for Smooth Muscle

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Antibody against QKI was purchased from Santa Cruz Biotech (sc-103851), USA. Antibodies against QKI5, QKI6, QKI7 were from Millipore (AB9904, AB9906, AB9908), USA. Antibody against Smooth Muscle Myosin Heavy Chain (SMMHC) was from AbD Serotec (Rabbit, AHP1117). Antibodies against SM22 (rabbit, Ab14106) and calponin (rabbit, Ab46794) were from Abcam, UK. Antibodies against GAPDH (mouse) and monoclonal anti-α smooth muscle actin (SMA) (Clone 1A4, A5228) were from Sigma. All secondary antibodies were from Santa Cruz Biotech.

Wu Y., Li Z., Yang M., Dai B., Hu F., Yang F., Zhu J., Chen T, & Zhang L. (2017). MicroRNA-214 regulates smooth muscle cell differentiation from stem cells by targeting RNA-binding protein QKI. Oncotarget, 8(12), 19866-19878.

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