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30 g needle

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The 30-G needle is a medical device used for the administration of injections. It features a thin, hollow needle with a 30-gauge diameter, designed for precise and minimally invasive subcutaneous or intramuscular injections.

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Lab products found in correlation

Fast green, by Merck Group (2 mentions) Tweezer type electrodes, by Nepa Gene (2 mentions) Cuy21edit, by Nepa Gene (2 mentions)

2 protocols using 30 g needle

1

In Utero Electroporation of Somatosensory Cortex

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Standard bipolar in utero electroporation of the somatosensory cortex was performed as previously described (Bony et al., 2013 (link); Szczurkowska et al., 2016 (link)). Timed-pregnant Sprague Dawley rats (Harlan Italy SRL, Correzzana, Italy) were anaesthetized at E17.5 with isoflurane (induction, 3.5%; surgery, 2.5%), and uterine horns were exposed by laparotomy. Expression vectors (1–2 μg μl−1/Vector in water) and dye Fast Green (0.3 mg ml−1; Sigma, St. Louis, MO) were injected (5–6 μl) through the uterine wall into one of the embryo’s lateral ventricle by a 30-G needle (Pic indolor, Grandate, Italy). Each embryo’s head was held between tweezer-type electrodes (10 mm diameter; Nepa Gene, Chiba, Japan) across the uterus and five electrical pulses (amplitude, 50 V; duration, 50 ms; intervals, 100 ms) were delivered with a square-wave electroporation generator (CUY21EDIT; Nepa Gene). Uterine horns were returned into the abdominal cavity, and embryos continued their normal development.
Costa R.O., Martins H., Martins L.F., Cwetsch A.W., Mele M., Pedro J.R., Tomé D., Jeon N.L., Cancedda L., Jaffrey S.R, & Almeida R.D. (2019). Synaptogenesis Stimulates a Proteasome-Mediated Ribosome Reduction in Axons. Cell reports, 28(4), 864-876.e6.
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2

In Utero Electroporation of Somatosensory Cortex

Check if the same lab product or an alternative is used in the 5 most similar protocols
Standard bipolar in utero electroporation of the somatosensory cortex was performed as previously described (Bony et al., 2013 (link); Szczurkowska et al., 2016 (link)). Timed-pregnant Sprague Dawley rats (Harlan Italy SRL, Correzzana, Italy) were anaesthetized at E17.5 with isoflurane (induction, 3.5%; surgery, 2.5%), and uterine horns were exposed by laparotomy. Expression vectors (1–2 μg μl−1/Vector in water) and dye Fast Green (0.3 mg ml−1; Sigma, St. Louis, MO) were injected (5–6 μl) through the uterine wall into one of the embryo’s lateral ventricle by a 30-G needle (Pic indolor, Grandate, Italy). Each embryo’s head was held between tweezer-type electrodes (10 mm diameter; Nepa Gene, Chiba, Japan) across the uterus and five electrical pulses (amplitude, 50 V; duration, 50 ms; intervals, 100 ms) were delivered with a square-wave electroporation generator (CUY21EDIT; Nepa Gene). Uterine horns were returned into the abdominal cavity, and embryos continued their normal development.
Costa R.O., Martins H., Martins L.F., Cwetsch A.W., Mele M., Pedro J.R., Tomé D., Jeon N.L., Cancedda L., Jaffrey S.R, & Almeida R.D. (2019). Synaptogenesis Stimulates a Proteasome-Mediated Ribosome Reduction in Axons. Cell reports, 28(4), 864-876.e6.
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