For the ELIspot for granzyme B, the BD™ ELISPOT Human Granzyme B ELISPOT Set (BD Bioscience, San Diego, CA, USA) was used according to the manufacturer’s instructions. Patients’ PBMC were stimulated with 100 mg/mL MUC-1 (self-made as described in [44 (link)]) or 100 U/mL CA19.9 (Merck, Schwalbach/TS, Germany), or with the medium as a control. Spots were counted after 24 h of incubation using the KSELISPOT System, release 4.1 (Carl Zeiss Light Microscopy, Göttingen, Germany). Cells stimulated with the medium without the MUC-1 peptide served as controls.
Elispot human granzyme b elispot set
Manufactured by BD
Sourced in United States
The BD™ ELISPOT Human Granzyme B ELISPOT Set is a laboratory equipment used to detect and quantify human granzyme B-secreting cells. It provides a standardized platform for the measurement of cellular immune responses.
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Lab products found in correlation
2 protocols using elispot human granzyme b elispot set
1
ELISpot Assay for Granzyme B
2
Granzyme B ELISPOT Assay for MUC1 and CA19.9
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For analysis of granzyme B release the BD ELISPOT Human Granzyme B ELISPOT Set (BD Bioscience, San Jose, CA, USA) was used according to the manufacturer's instructions. Patients' PBMCs were stimulated with 100 μg ml−1 MUC1 – made as described in Schmidt et al (2007) (link) – or 100 U ml−1 CA19.9 (Merck, Schwalbach, Germany), or with medium as a control. Spots were counted after 24 h of incubation using the KS ELISPOT System, release 4.1 (Carl Zeiss Light Microscopy, Jena, Germany). Cells stimulated with medium without the peptides served as controls.
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