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Anti rabbit ab conjugated to horseradish peroxidase

Manufactured by GE Healthcare
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Sourced in United Kingdom

The anti-rabbit antibody conjugated to horseradish peroxidase is a laboratory reagent used for immunodetection and immunoassays. It binds to rabbit primary antibodies and the horseradish peroxidase enzyme enables colorimetric or chemiluminescent detection.

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Lab products found in correlation

Supersignal enhanced chemiluminescence system, by Thermo Fisher Scientific (2 mentions) β actin, by Merck Group (2 mentions) Phospho p65 s536, by Abcam (1 mentions)

2 protocols using anti rabbit ab conjugated to horseradish peroxidase

1

Western Blot Analysis of OPRS1/Sigmar-1

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Cells were lysed in Laemmli buffer and the protein extracts were tested by Ab specific for OPRS1/Sigmar-1 (Abcam, Cambridge, UK), and β-actin (Sigma) diluted at 1∶500 and 1∶000, respectively. Anti-rabbit Ab conjugated to horseradish peroxidase (GE Healthcare, Little Chalfont Buckinghamshire, UK) was used as the secondary Ab at a dilution of 1∶5000. The SuperSignal enhanced chemiluminescence system was used for probing target proteins (Thermo Scientific, Rockford, IL). After the membranes had been probed for OPRS1/Sigmar-1, they were stripped and re-probed for β-actin.
Szabo A., Kovacs A., Frecska E, & Rajnavolgyi E. (2014). Psychedelic N,N-Dimethyltryptamine and 5-Methoxy-N,N-Dimethyltryptamine Modulate Innate and Adaptive Inflammatory Responses through the Sigma-1 Receptor of Human Monocyte-Derived Dendritic Cells. PLoS ONE, 9(8), e106533.
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2

Western Blot Analysis of Cell Signaling

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Cells were lysed in Laemmli buffer and the protein extracts were tested by Ab specific for Sig-1R/OPRS1, HIF-1α, ATF6, p65, phospho-p65 (S536) (all from Abcam), and β-actin (Sigma, Schnelldorf, Germany) diluted at 1:500 and 1:000, respectively. Anti-rabbit Ab conjugated to horseradish peroxidase (GE Healthcare, Little Chalfont Buckinghamshire, UK) was used as the secondary Ab at a dilution of 1:5000. The SuperSignal enhanced chemiluminescence system was used for probing target proteins (Thermo Scientific, Rockford, IL). After the membranes had been probed for Sig-1R/OPRS1 or HIF-1α, they were stripped and re-probed for β-actin.
Szabo A., Kovacs A., Riba J., Djurovic S., Rajnavolgyi E, & Frecska E. (2016). The Endogenous Hallucinogen and Trace Amine N,N-Dimethyltryptamine (DMT) Displays Potent Protective Effects against Hypoxia via Sigma-1 Receptor Activation in Human Primary iPSC-Derived Cortical Neurons and Microglia-Like Immune Cells. Frontiers in Neuroscience, 10, 423.
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