Antibodies for Western blotting were as follow: AP2S1 (ab128950, Abcam, 1:1000), APP‐full length and CTFs (A8717, Sigma, 1:1000), BACE1 (ab2077, Abcam, 1:1000), sAPP‐β (SIG‐39138, Biolegend, 1:500), VPS41 (ab181078, Abcam, 1:1000), VPS16 (17776‐1‐AP, Proteintech, 1:1000), GAPDH (Proteintech, 1:10000). Horseradish peroxidase‐conjugated anti‐mouse (AB_2722565, 1:5000) and anti‐rabbit (AB_2722564, 1:5000) secondary antibodies were purchased from Proteintech (Wuhan, China). Antibodies for immunofluorescence included: APP (MAB348, Sigma, 1:200), Aβ (MOAB‐2, Novus Biologicals, 1:1000), EEA1 (#2411, 1:200), GM130 (#12480, 1:200), RAB9 (#5118, 1:200), LAMP1 (#9091, 1:100) were purchased from Cell Signaling Technology. Alexa Fluor 488‐labeled Goat Anti‐Rabbit (A0428, 1:200), Cy3‐l‐abeled Goat Anti‐Rabbit (A0516, 1:200) and Alexa Fluor 647‐labeled Goat Anti‐Mouse (A0473, 1:200) secondary antibodies were purchased from Beyotime (Shanghai, China). Pitstop2 (HY‐115604), dynasore (HY‐13863), chloroquine (CQ, HY‐17589A), bafilomycin A1 (BafA1, HY‐100558), β‐secretase inhibitor LY2811376 (HY‐10472), α‐secretase inhibitor GI254023X (HY‐19956) and γ‐secretase inhibitor DAPT (HY‐13027) were purchased from MCE (Monmouth Junction, NJ, USA).
Cy3 l abeled goat anti rabbit a0516
Manufactured by Beyotime
Sourced in China
Cy3-l-abeled Goat Anti-Rabbit (A0516) is a secondary antibody conjugated with the Cy3 fluorescent dye. It is designed for the detection and quantification of rabbit primary antibodies in various immunoassay applications.
Automatically generated - may contain errors
Lab products found in correlation
Lamp1, by Cell Signaling Technology (1 mentions)
A8717, by Merck Group (1 mentions)
Ab128950, by Abcam (1 mentions)
Sapp β sig 39138, by BioLegend (1 mentions)
Ab181078, by Abcam (1 mentions)
Gapdh, by Proteintech (1 mentions)
Ab109012, by Abcam (1 mentions)
Alexa fluor 488 labeled goat anti mouse a0428, by Beyotime (1 mentions)
Ab179515, by Abcam (1 mentions)
Ab207612, by Abcam (1 mentions)
Ab215203, by Abcam (1 mentions)
Ab127537, by Abcam (1 mentions)
Ab208943, by Abcam (1 mentions)
Ab4207, by Abcam (1 mentions)
2 protocols using cy3 l abeled goat anti rabbit a0516
1
Western Blot and Immunofluorescence Antibodies
2
Pyroptosis Compound Library Identification
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Pyroptosis Compound Library (L7400) were purchased from Selleck (Houston, TX, United States). Primary antibodies against NLRP3 (ab4207), GSDMD N-terminus (GSDMD-N; ab215203), caspase-1 (ab179515), LAMP-1 (ab208943), ASC (ab127537), P62 (ab109012), and beclin 1 (ab207612) were obtained from Abcam (Cambridge, MA, United States). The anti-light chain 3 (LC3) primary antibody (FNab04716) was obtained from Abcam Fine Test (Beijing, China). The horseradish peroxidase-conjugated secondary antibody (SA00001-1) and anti-glyceraldehyde-3-phosphate dehydrogenase antibody (60004-1-1g) were purchased from Proteintech (Wuhan, China). The following immunoglobulinG (H + L) antibodies were purchased from Beyotime Biotechnology (Shanghai, China): Cy3-labeled goat anti-rabbit (A0516), Cy3-labeled goat anti-mouse (A0521), Alexa Fluor 488-labeled goat anti-rabbit (A0423), and Alexa Fluor 488-labeled goat anti-mouse (A0428).
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