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Type 9 ultra low melt agarose

Manufactured by Merck Group
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Type IX ultra‐low melt agarose is a laboratory grade agarose product. It is designed for applications requiring low gelling and melting temperatures.

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Lab products found in correlation

Em pact2, by Leica (1 mentions)

Close spelling variants of this product

Low-melt agarose Type IX Agarose

2 protocols using type 9 ultra low melt agarose

1

High-Pressure Freezing of Auxin-Treated Cells

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Sapphire disks were coated with a 10 nm carbon layer followed by collagen (Corning) coating per the manufacturer's instructions. Coated disks were sterilized under UV light and transferred to new sterile 3 cm dishes for plating the cells. After reaching 80%–100% confluence, cells were equilibrated in fresh media for 2–3 h at 37°C, treated with Auxin various times. HPF at specified time points was performed in cryo‐protectant (PBS with 2% Type IX ultra‐low melt agarose [Sigma‐Aldrich], 100 mM d‐mannitol, and 2% FBS) using a Leica EM PACT2 high‐pressure freezing unit (Leica Microsystems) with rapid transfer system at high pressure (2100 bar). All solutions, bayonets, and sample holders were pre‐warmed to 37°C, all manipulations were carried out on a 37°C heating platform.
Sumya F.T., Pokrovskaya I.D., D'Souza Z, & Lupashin V.V. (2022). Acute COG complex inactivation unveiled its immediate impact on Golgi and illuminated the nature of intra‐Golgi recycling vesicles. Traffic (Copenhagen, Denmark), 24(2), 52-75.
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2

Cryo-preservation of Auxin-treated Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sapphire disks were coated with a 10 nm carbon layer followed by collagen (Corning) coating per the manufacturer’s instructions. Coated disks were sterilized under UV light and transferred to new sterile 3 cm dishes for plating the cells. After reaching 80%–100% confluence, cells were equilibrated in fresh media for 2–3 h at 37°C, treated with Auxin various times. HPF at specified time points was performed in cryo-protectant (PBS with 2% Type IX ultra-low melt agarose [Sigma-Aldrich], 100 mM d-mannitol, and 2% FBS) using a Leica EM PACT2 high-pressure freezing unit (Leica Microsystems) with rapid transfer system at high pressure (2100 bar). All solutions, bayonets, and sample holders were pre-warmed to 37°C, all manipulations were carried out on a 37°C heating platform.
Sumya F.T., Pokrovskaya I.D., D'Souza Z, & Lupashin V.V. (2022). Acute COG complex inactivation unveiled its immediate impact on Golgi and illuminated the nature of intra‐Golgi recycling vesicles. Traffic (Copenhagen, Denmark), 24(2), 52-75.
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