Hrp labeled donkey anti rabbit igg secondary antibody

Manufactured by Jackson ImmunoResearch

Sourced in United States

HRP labeled Donkey Anti-Rabbit IgG secondary antibody is a laboratory reagent used for the detection of rabbit primary antibodies in various immunoassays. It is conjugated with horseradish peroxidase (HRP) enzyme, which enables colorimetric or chemiluminescent detection upon substrate addition.

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Lab products found in correlation

Pierce bca protein quantification kit, by Thermo Fisher Scientific (2 mentions) Nickel his gravitrap affinity column, by GE Healthcare (2 mentions) Gravitrap, by Cytiva (2 mentions) Rabbit anti her2 antibody clone ep3, by ZSGB-BIO (2 mentions) Recombinant human her2 erbb2 protein, by Sino Biological (2 mentions) Mouse anti ki67 antibody clone mib1, by ZSGB-BIO (1 mentions) Bca protein quantification kit, by Thermo Fisher Scientific (1 mentions) Ventana anti her2 neu 4b5 rabbit monoclonal primary antibody, by Roche (1 mentions) Rabbit anti cyclind1 monoclonal antibody ep12, by ZSGB-BIO (1 mentions)

Spelling variants of this product

Anti-rabbit secondary antibody (44 citations) Donkey anti-rabbit HRP (38 citations) HRP-labeled secondary antibodies (25 citations) Donkey anti-rabbit secondary antibody (14 citations) Rabbit anti-HRP (12 citations) Secondary HRP antibodies (6 citations) Donkey anti-rabbit IgG secondary antibody (5 citations) Donkey-anti-rabbit-HRP antibodies (5 citations) Donkey anti-rabbit HRP secondary antibody (2 citations) HRP-labeled anti-rabbit antibody (2 citations)

4 protocols using hrp labeled donkey anti rabbit igg secondary antibody

Protein Expression and Purification

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The general reagents for cell culture were purchased from Thermo Fisher Scientifics . The chemicals used for protein expression were purchased from Takara Inc., and the Nickel‐His GraviTrap affinity column for protein purification was purchased from GE Healthcare. All the other chemicals were purchased from Sinopharm Chemicals.
Both Mouse anti‐Ki67 antibody (clone MIB1) and Rabbit anti‐cyclinD1 antibody (clone EP12) were purchased from ZSGB‐BIO. HRP‐labeled Donkey Anti‐Rabbit IgG secondary antibody was purchased from Jackson Immunoresearch lab. Pierce BCA protein quantification kit was purchased from Thermo Fisher Scientific Inc. Recombinant human Ki67 and cyclinD1 proteins were prepared in the house. QDB plate was manufactured by Quanticision Diagnostics Inc. at RTP, NC, USA.

Yu G., Lyu J., Li Y., Zhang Y., Lyu Y., Zhang W., Zhang J., Cai B., Zhang J, & Tang F. (2022). Validation of absolutely quantitated Ki67 and cyclinD1 protein levels for prognosis of Luminal‐like breast cancer patients. Journal of Clinical Laboratory Analysis, 36(8), e24601.

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HER2 Protein Immunodetection Protocol

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All of the chemicals were purchased from Sinopharm Chemicals (Beijing, P. R. China). Recombinant human HER2/ErbB2 protein was purchased from Sino Biological Inc. (Beijing, China). Ventana anti-HER2/neu (4B5) rabbit monoclonal primary antibody was purchased from Roche Diagnostics GmbH. Rabbit anti-HER2 antibody (clone EP3) was purchased from ZSGB-BIO (Beijing, China). HRP labeled Donkey Anti-Rabbit IgG secondary antibody was purchased from Jackson Immunoresearch lab (West Grove, PA, USA). BCA protein quantification kit was purchased from Thermo Fisher Scientific Inc. (Calsband, CA, USA). QDB plate was provided by Quanticision Diagnostics, Inc. (RTP, USA).

Yu G., Zhang W., Zhang Y., Lv J., Wu S., Sui X., Zhang J, & Tang F. (2020). Developing a routine lab test for absolute quantification of HER2 in FFPE breast cancer tissues using Quantitative Dot Blot (QDB) method. Scientific Reports, 10, 12502.

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Quantification of Breast Cancer Biomarkers

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Recombinant human Her-2/ErbB2 protein (cat# 10004-H20B1) was purchased from Sino
Biological Inc. ER, PR, and Ki67 recombinant proteins were purified in the
house. QDB plate was purchased from Quanticision Diagnostics, Inc (RTP). Anti-PR
(clone 1E2, cat# 790-4296) rabbit monoclonal primary antibody was purchased from
Roche Diagnostics GmbH. Rabbit anti-ER (clone SP1, cat# ab13370) antibody was
purchased from Abcam Inc. Rabbit anti-Her2 antibody (clone EP3, cat# ZA-0023)
and mouse anti-Ki67 (clone MIB1, cat# ZM-0167) were purchased from ZSGB-BIO
(www.zsbio.com). HRP labeled Donkey Anti-Rabbit IgG secondary
antibody was purchased from Jackson Immunoresearch lab (West Grove). BCA total
protein quantification kit was purchased from Thermo Fisher Scientific Inc
(Calsband).

Lyv J., Yu G., Zhang Y., Lyv Y., Zhang W., Zhang J, & Tang F. (2021). 3D Visualization of the Dynamic Bidirectional Talk Between ER/PR and Her2 Pathways. Technology in Cancer Research & Treatment, 20, 15330338211065603.

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Cyclin D1 Protein Expression and Analysis

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The general reagents for cell culture were purchased from Thermo Fisher Scientifics (Waltham, MA, USA). The chemicals used for protein expression were purchased from Takara Inc. (Dalian, China), and Nickel-His GraviTrap affinity column for protein purification was purchased from GE Healthcare. All the other chemicals were purchased from Sinopharm Chemicals (Beijing, P. R. China).
Rabbit anti-cyclin D1 monoclonal antibody (EP12) was purchased from ZSGB-BIO (Beijing, China). HRP labeled Donkey Anti-Rabbit IgG secondary antibody was purchased from Jackson Immunoresearch lab (Pike West Grove, PA, USA). Pierce BCA protein quantification kit was purchased from Thermo Fisher Scientific Inc.
(Calsband, CA, USA). Recombinant human cyclin D1 protein was prepared in the house. QDB plate was manufactured by Quanticision Diagnostics Inc at RTP, NC, USA.

Hao J., Zhang W., Lv Y., Zou J., Zhang Y., Lv J., Xie S., Zhang C., Zhang J., & Tang F. (2020). Combined use of absolutely quantitated cyclin D1 and Ki67 protein levels to improve prognosis of Luminal-like breast cancer.

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