Bx51 epifluorescent light microscope

Manufactured by Olympus

Sourced in Japan

The BX51 is an epifluorescent/light microscope manufactured by Olympus. It is designed for high-quality microscopic imaging and analysis. The core function of the BX51 is to provide clear, detailed images of specimens using both fluorescent and transmitted light techniques.

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Lab products found in correlation

Newcast software, by Visiopharm (2 mentions) Axiovision software, by Zeiss (1 mentions)

Spelling variants of this product

BX51 microscope (3784 citations) BX51 light microscope (520 citations) Epifluorescent microscope (29 citations) BX51 epifluorescent microscope (18 citations)

3 protocols using bx51 epifluorescent light microscope

Quantifying Neurogenesis and Cell Markers

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Cells were
visualized/quantified
using an Olympus BX51 epifluorescent/light microscope (Olympus, Japan)
and the stereology NewCAST software (Visiopharm, Denmark). However,
the stereology option was not applied due to the low number of cells.
Instead, microscope real-time images were displayed on the screen,
and the total number of cells per section was quantified and adjusted
on the area (1 mm²). In MOB, BrdU- (density per 1 mm²) and BrdU/NeuN-positive (%) cells were quantified on three
coronal sections (from 4.28 to 3.92 mm in the distance from Bregma).
PC volume measurement and quantification of total number of DCX-positive
cells in PC was done on 11 coronal sections per animal (from 1.70
to −2.70 mm in the distance from Bregma). For other markers
(calbindin, somatostatin, and NeuN/cFos), cell density per 1 mm² in PC was calculated on six coronal sections (1.10, 0.14,
−0.82, −1.22, −1.82, and −2.46 mm distance
from Bregma). Quantifications were performed by persons blinded to
experimental groups and outcome assessment.

Lietzau G., Nyström T., Wang Z., Darsalia V, & Patrone C. (2020). Western Diet Accelerates the Impairment of Odor-Related Learning and Olfactory Memory in the Mouse. ACS Chemical Neuroscience, 11(21), 3590-3602.

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Quantifying Neuronal Markers in Cerebellum

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Immunoreactive cells were counted using a computerized setup for stereology, equipped with NewCast software (Visiopharm, Hoersholm, Denmark), connected to Olympus BX51 epifluorescent/light microscope (Olympus, Tokyo, Japan). The number of NeuN-, Calbindin-D28k- and pJNK-positive cells in the PC was quantified on three evenly spaced sections (400μm) in each animal starting at 0.70mm from Bregma (Figure 1). Quantifications were performed using the optical fractionator method [24 , 58 (link)]. Number of neurites in the Calbindin-D28k-positive cells was estimated in 50-60 randomly selected cells up to the fifth level of branching, e.g. the neurites protruding from cell body were marked as level 1 and every consecutive branching from the level 1 neurites - as level 2 and so on. The average number of neurites per cell was calculated.

Lietzau G., Nyström T., Östenson C.G., Darsalia V, & Patrone C. (2016). Type 2 diabetes-induced neuronal pathology in the piriform cortex of the rat is reversed by the GLP-1 receptor agonist exendin-4. Oncotarget, 7(5), 5865-5876.

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Quantifying Neuronal Cell Populations

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The DARPP-32-, GAD67-, GFAP-, Iba-1-, PV-, and TH-positive cells were quantified using a computerized stereology toolbox equipped with Visiopharm v. 4.2.1.0 software for digital image analysis (NewCast, Denmark), connected to Olympus BX51 epifluorescent/light microscope (Olympus, Japan). Olig2-, GPR17-, PCNA-and GSTπ-positive cells were quantified using an inverted fluorescence microscope (200M; Zeiss, Milan, Italy) connected to a PC computer equipped with Axiovision software (Zeiss). In striatum, positive cells were counted on three coronal sections per animal (1.50, 0.00 and -1.00 mm distance to Bregma).
Quantification of TH+ cells in substantia nigra pars compacta was done on three sagittal sections per animal (1.40, 2.10 and 2.60 mm distance to Bregma). The cell density per 1 mm 2 for all the IHC markers was determined. Mean volume (in µm 3 ) of Iba-1+ and PV+ cells was measured, using nucleator technique (Gundersen et al., 1988) , by Visiopharm software.
Experiments were performed by persons blinded to group assignment and outcome assessment.

Lietzau G., Magni G., Kehr J., Yoshitake T., Candeias E., Duarte A.I., Pettersson H., Skogsberg J., Abbracchio M.P., Klein T., Nyström T., Ceruti S., Darsalia V, & Patrone C. (2020). Dipeptidyl peptidase-4 inhibitors and sulfonylureas prevent the progressive impairment of the nigrostriatal dopaminergic system induced by diabetes during aging. Neurobiology of aging, 89.

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