At the end of the experiment, mice were sacrificed and blood samples (PBS, L-DOX, H-DOX, L-DOX@NPs and H-DOX@NPs) were collected by intracardiac puncture. Then, the plasma samples were obtained by centrifugation (5000 rpm, 4°C, 30 min), and the levels of aspartate aminotransferase (AST), blood urea nitrogen (BUN), creatine kinase (CK) and lactate dehydrogenase (LDH) were measured using Cobas 6000 automatic biochemical analyzer (Roche, Switzerland).
Cobas 6000 automatic biochemical analyzer
Manufactured by Roche
Sourced in Switzerland, China
The Cobas 6000 is an automatic biochemical analyzer designed for clinical laboratories. It is capable of performing various biochemical tests and analyses on patient samples. The core function of the Cobas 6000 is to automate the processes of sample handling, reagent management, and analytical testing in order to provide accurate and reliable results.
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Lab products found in correlation
2 protocols using cobas 6000 automatic biochemical analyzer
1
Biochemical Analysis of Plasma Biomarkers
2
Biochemical and Antioxidant Serum Assay
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Biochemical index and enzyme activity were measured after the collected, frozen serum samples finished the two-step gradient thawing. Alanine aminotransferase (ALT), aspartate transaminase (AST), total protein (TP), alkaline phosphatase (ALP), and urea were all determined by a Cobas 6000 automatic biochemical analyzer (Roche Diagnostics Co., Ltd., Shanghai, China). The enzyme activities of glutathione peroxidase (GSH-Px), diamine oxidase (DAO), and also the malonaldehyde (MDA) concentration of serum were measured by colorimetric methods with a T9CS+ spectrophotometer (Purkinje General Instrument Co., Ltd., Beijing, China). A microplate reader detected the total antioxidant capacity (T-AOC) and superoxide dismutase (SOD). All the antioxidant indexes aforementioned were conducted according to the manufacturer's instructions.
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