Mouse igg1 k isotype control apc

Manufactured by Thermo Fisher Scientific

The Mouse IgG1 K Isotype Control (APC) is a laboratory reagent used as a control in flow cytometry experiments. It consists of mouse immunoglobulin G1 (IgG1) antibodies conjugated with the fluorescent dye allophycocyanin (APC). This control is used to distinguish specific antibody binding from nonspecific background signal in flow cytometry analyses.

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Lab products found in correlation

Anti human cd45 fitc, by Thermo Fisher Scientific (1 mentions) Mouse igg1k isotype control, by Thermo Fisher Scientific (1 mentions) Anti human cd4 percp cyanine5, by Thermo Fisher Scientific (1 mentions) Anti human cd19 apc, by Thermo Fisher Scientific (1 mentions) Anti human cd3 apc efluor780, by Thermo Fisher Scientific (1 mentions) Anti mouse cd45 efluor450, by Thermo Fisher Scientific (1 mentions) Lsrfortessa, by BD (1 mentions)

2 protocols using mouse igg1 k isotype control apc

Neutrophil Lipid Raft and CD11b Expression

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Following incubation with LPS, neutrophils were washed with PBS and incubated with FACS buffer [PBS containing 0.25 % bovine serum albumin (BSA), and 2.1 mM EDTA] for 15 min at room temperature. Neutrophils were again washed and incubated in FACS buffer with 1 μg/ml FITC-cholera toxin B (Sigma) for 30 min at room temperature. Mouse anti-human CD11b (activation epitope) allophycocyanin (APC; eBioscience) was added to samples and neutrophils were further incubated for 30 min at 4 °C. After washing with PBS, the cells were fixed in 2 % paraformaldehyde. In control experiments, cells were treated in the presence and absence of mouse IgG1 K Isotype Control (APC, eBioscience). CD11b and lipid raft expressions were measured by flow cytometry using BD LRSII (Becton Dickinson). Analysis was performed using FlowJo software (version 7.6.5, Tree Star, Ashland, OR). Results were normalized to un-stimulated saline-treated control and expressed as a percentage change compared to control.

Sharifov O.F., Xu X., Gaggar A., Tabengwa E.M., White C.R., Palgunachari M.N., Anantharamaiah G.M, & Gupta H. (2014). L-4F Inhibits Lipopolysaccharide-Mediated Activation of Primary Human Neutrophils. Inflammation, 37(5), 1401-1412.

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Multicolor Flow Cytometry for Human Cell Engraftment in Humanized Mice

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To detect human cells engrafted in HUMAMICE, multi-colors cytometric analysis was performed using BD LSR Fortessa, according to the manufacturer’s protocol but with a minor modification. HUMAMICE were sacrificed and the spleens were removed at three different times after hPBMCs were transplanted. Splenocytes were collected and lysed by ACK lysis buffer, counted and incubated with an appropriate volume of antibodies for 1 hour at 4°C and then subjected to flow cytometry analysis.
Commonly used antibodies included: Anti-Human CD45 FITC, Clone: 2D1, (ebioscience9011‐9459); isotype control, Mouse IgG1 K Isotype Control (ebioscience, FITC 11–4714) ; Anti-Mouse CD45 eFluor^® 450 (ebioscience, 48-0451-82) ; isotype control, Mouse IgG2b K Isotype Control eFluor^® 450 (ebioscience, 48-4732-82) ; Anti-Human CD3 APC-eFluor^® 780, Clone: UCHT1, (ebioscience, 47–0038) ; Mouse IgG1 K Isotype Control APC- eFluor^® 780 (ebioscience, 47–4714) ; Anti-Human CD4 PerCP-Cyanine5.5, Clone: OKT4 (OKT-4), (ebioscience, 45–0048); isotype control, Mouse IgG2b K Isotype Control PerCP-Cyanine5.5 (cat. 45–4732) ; Anti-Human CD8a PE-Cyanine7, Clone: SK1, (ebioscience, 25–0087); isotype control, Mouse IgG1 K Isotype Control PE-Cyanine7 (ebioscience, 25–4714) ; Anti-Human CD19 APC, Clone: 2H7, (ebioscience, 17–0209); isotype control, Mouse IgG1 K Isotype Control APC (ebioscience, 17–4714).

Zeng Y., Liu B., Rubio M.T., Wang X., Ojcius D.M., Tang R., Durrbach A., Ru Z., Zhou Y, & Lone Y.C. (2017). Creation of an immunodeficient HLA-transgenic mouse (HUMAMICE) and functional validation of human immunity after transfer of HLA-matched human cells. PLoS ONE, 12(4), e0173754.

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