The following antibodies were used: Rabbit Phospho-histone H3 (Cell Signalling #9701, 1:200), rabbit anti-Engrailed (Santa Cruz Biotechnology d-300; 1:200), goat anti-GFP-FITC (Abcam ab6556, 1:500), guinea pig anti-Sqh-1P (1:100, (Zhang and Ward, 2011 (link))) (called MRLC-1P in this paper), mouse anti-phospho-Tyrosine (Cell signaling #9411; 1:1000), mouse anti-Wingless (DSHB 4D4; 1:50); mouse anti-Dlg (DSHB 4F3; 1:500) Rabbit anti-Pins (Izumi et al., 2006 (link)) (1:1000, a gift from F. Matsuzaki), rabbit anti-Mud(Izumi et al., 2006 (link)) (1:200, a gift from F. Matsuzaki). Secondary antibodies conjugated to fluorescent dyes were obtained from Jackson ImmunoResearch Laboratories, Invitrogen and Life Technologies. Cell nuclei were stained using DAPI (Sigma-Aldrich).
Rabbit anti engrailed
Manufactured by Santa Cruz Biotechnology
Rabbit anti-Engrailed is a primary antibody that recognizes the Engrailed protein, a highly conserved homeodomain transcription factor involved in embryonic development and cell fate determination. This antibody is produced in rabbits and can be used to detect and study the expression and localization of Engrailed in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti phospho tyrosine, by Cell Signaling Technology (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Goat anti gfp fitc, by Abcam (1 mentions)
Anti rabbit alexa647, by Thermo Fisher Scientific (1 mentions)
Anti mouse alexa 488, by Thermo Fisher Scientific (1 mentions)
Anti rabbit cy3, by Jackson ImmunoResearch (1 mentions)
Anti mouse cy5, by Jackson ImmunoResearch (1 mentions)
Anti mouse cy3, by Jackson ImmunoResearch (1 mentions)
Anti chicken fitc, by Jackson ImmunoResearch (1 mentions)
Dylight 405, by Thermo Fisher Scientific (1 mentions)
Uas mcd8 gfp, by Bloomington Drosophila Stock Center (1 mentions)
Vectashield, by Vector Laboratories (1 mentions)
Streptavidin with alexa fluor 405 conjugate, by Thermo Fisher Scientific (1 mentions)
3 protocols using rabbit anti engrailed
1
Immunofluorescence Staining Protocol
2
Drosophila Neuronal Development Assay
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The following fly stocks were used: OregonR (wild type), elavC155-Gal4 [17 (link)], hs-mFlp5MH12, UAS-FB1.1B260b [18 (link)] (both from Iris Salecker), eveRRK-Gal4 [19 (link)] (from Matthias Landgraf), UAS-mCD4::tdGFP, UAS-mCD8::GFP (both from Bloomington Drosophila Stock Center), gooseberry-distal-lacZ (from Fernando Diaz-Benjumea); gsb-Gal4>UAS-mCD8::GFP (from Christian Berger).
Primary antibodies used were: chicken-anti-Beta-Gal (1:1000; Abcam, #ab9361); rabbit-anti-Even skipped (1:1000) [20 (link)] (from Manfred Frasch,), mouse-anti-Even skipped (1:2; DSHB), rabbit-anti-GFP (1:250; Torrey Pines Biolabs), mouse-BP102 (1:20; DSHB), mouse-anti-Futsch (1:20; DSHB), rabbit-anti-Engrailed (1:100, Santa Cruz), mouse-anti-engrailed (1:2; DSHB), mouse-anti-ladybird (1:2, [21 (link)], from Krzysztof Jagla).
The fluorescent secondary antibodies used (all diluted 1:500, except DyLight 405: 1:250) were: anti-mouse-Alexa 647, anti-mouse-Alexa 488, anti-mouse Alexa 568, anti-rabbit -Alexa 647, ant-rabbit-Alexa 568 (all Invitrogen); anti-chicken-Cy5, anti-rabbit-Cy3, anti mouse-Cy3, anti-mouse-Cy5, anti-rabbit-FITC, anti-chicken-FITC (all Jackson Immunoresearch Laboratories), anti-rabbit-DyLight 405 (Dianova).
Primary antibodies used were: chicken-anti-Beta-Gal (1:1000; Abcam, #ab9361); rabbit-anti-Even skipped (1:1000) [20 (link)] (from Manfred Frasch,), mouse-anti-Even skipped (1:2; DSHB), rabbit-anti-GFP (1:250; Torrey Pines Biolabs), mouse-BP102 (1:20; DSHB), mouse-anti-Futsch (1:20; DSHB), rabbit-anti-Engrailed (1:100, Santa Cruz), mouse-anti-engrailed (1:2; DSHB), mouse-anti-ladybird (1:2, [21 (link)], from Krzysztof Jagla).
The fluorescent secondary antibodies used (all diluted 1:500, except DyLight 405: 1:250) were: anti-mouse-Alexa 647, anti-mouse-Alexa 488, anti-mouse Alexa 568, anti-rabbit -Alexa 647, ant-rabbit-Alexa 568 (all Invitrogen); anti-chicken-Cy5, anti-rabbit-Cy3, anti mouse-Cy3, anti-mouse-Cy5, anti-rabbit-FITC, anti-chicken-FITC (all Jackson Immunoresearch Laboratories), anti-rabbit-DyLight 405 (Dianova).
3
Immunostaining of Drosophila Embryos
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Embryos were fast fixed at the interface between 37% formaldehyde and 100% heptane for 8 minutes then washed thoroughly in PBS-TX. The vitelline membrane was manually removed through physical manipulation with a tungsten needle. Embryos were blocked in PBS-TX-BSA for 30mins at RT. Embryos were incubated with primary antibodies in blocking solution overnight at 4 o C. Excess antibody was removed by washing embryos thoroughly in PBS-TX.
Embryos were incubated with secondary antibodies in blocking solution for 1 hour at RT.
Excess antibodies were removed by washing thoroughly in PBS-TX. Stained embryos were stored in Vectashield (Vectorlabs) until mounted.
Primary antibodies used were: mouse anti-phospho-Tyrosine (Cell signaling #9411; 1:100), rabbit anti-Engrailed (Santa Cruz D300), chick anti-Beta-gal (Abcam ab9361), rabbit anti-Tartan (Chang et al., 1993) (kind gift of Shigeo Hayashi), rabbit anti-comm (Tear et al., 1996) (kind gift of Guy Tear).
Secondary antibodies conjugated to fluorescent dyes were obtained from Jackson ImmunoResearch Laboratories, Invitrogen and Life Technologies. Streptavidin with Alexa
Fluor 405 conjugate was from ThermoFisher Scientific.
Embryos were incubated with secondary antibodies in blocking solution for 1 hour at RT.
Excess antibodies were removed by washing thoroughly in PBS-TX. Stained embryos were stored in Vectashield (Vectorlabs) until mounted.
Primary antibodies used were: mouse anti-phospho-Tyrosine (Cell signaling #9411; 1:100), rabbit anti-Engrailed (Santa Cruz D300), chick anti-Beta-gal (Abcam ab9361), rabbit anti-Tartan (Chang et al., 1993) (kind gift of Shigeo Hayashi), rabbit anti-comm (Tear et al., 1996) (kind gift of Guy Tear).
Secondary antibodies conjugated to fluorescent dyes were obtained from Jackson ImmunoResearch Laboratories, Invitrogen and Life Technologies. Streptavidin with Alexa
Fluor 405 conjugate was from ThermoFisher Scientific.
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