LNCaP, PC3, and DU145 cells were purchased from ATCC and maintained at 37°C with 5% CO2 in RPMI (Wako) supplemented with 10% fetal bovine serum (FBS), 20 U/ml penicillin, and 100 µg/ml streptomycin. HEK293T cells were maintained at 37°C with 5% CO2 in Dulbecco's modified Eagle medium (Wako) supplemented with 10% FBS, 20 U/ml penicillin, and 100 µg/ml streptomycin. Human umbilical vascular endothelial cells (HUVECs) were purchased from Lonza. HUVECs were maintained at 37°C with 5% CO2 in EBM‐2 (Lonza) according to the manufacturer's instructions. HUVECs at passages 2–4 were used for the experiments. HUVECs cultured on collagen I gels were treated with the CM. PC3 cells that stably express Myc‐PSMA or PSMA‐myc were established by selection with 50 µg/ml blasticidin S (Wako) after infection with lentivirus carrying the Myc‐PSMA or PSMA‐myc gene.
Human umbilical vascular endothelial cells huvecs
Manufactured by Lonza
Sourced in Switzerland
Human umbilical vascular endothelial cells (HUVECs) are primary cells derived from the blood vessels of the human umbilical cord. They are a commonly used in vitro model for the study of vascular endothelial cell biology.
Automatically generated - may contain errors
Lab products found in correlation
Roswell park memorial institute (rpmi), by Fujifilm (1 mentions)
Dulbecco s modified eagle medium, by Fujifilm (1 mentions)
Blasticidin s, by Fujifilm (1 mentions)
Ebm 2, by Lonza (1 mentions)
Huvecs, by Lonza (1 mentions)
Human epidermal growth factor (hegf), by Lonza (1 mentions)
Dabigatran, by Merck Group (1 mentions)
2 7 dichlorodihydrofluorescein diacetate h2dcfda, by Thermo Fisher Scientific (1 mentions)
Trypsin neutralizing solution, by Lonza (1 mentions)
Hfgf b, by Lonza (1 mentions)
Rivaroxaban, by Merck Group (1 mentions)
Trypsin with edta, by Lonza (1 mentions)
25 hydroxycholesterol 25 ohc, by Merck Group (1 mentions)
R3 igf 1, by Lonza (1 mentions)
Ascorbic acid, by Lonza (1 mentions)
Ga 1000, by Lonza (1 mentions)
2 protocols using human umbilical vascular endothelial cells huvecs
1
Cell Culture and Lentiviral Transduction Protocols
2
HUVEC Endothelial Cell Culture Protocol
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Human umbilical vascular endothelial cells (HUVECs), trypsin with EDTA, trypsin neutralizing solution, endothelial cell growth medium-2 (EGM-2) with hydrocortisone, hFGF-B, vascular endothelial growth factor (VEGF), R3-IGF-1, ascorbic acid, hEGF, GA-1000, heparin and fetal bovine serum (FBS), were purchased in Lonza (Basel, Switzerland). Endonuclease III (Nth) and human 8-oxoguanine DNA glycosylase (hOGG1) were acquired in New England Biolabs (Ipswich, MA, USA). 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA) was purchased from Invitrogen (Waltham, Massachusetts, USA). Low melting point (LMP), normal melting point (NMP) agarose, 4′,6-diamidino-2-phenylindole (DAPI), dabigatran, rivaroxaban and 25-hydroxycholesterol (25-OHC) were bought from Sigma-Aldrich (St. Louis, MO, USA) while other chemicals were purchased from Carl Roth GmbH + Co. KG (Karlsruhe, Germany) and POCh (Gliwice, Poland) and were of analytical grade.
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