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Myc tag 2276

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Myc-Tag (2276) is a laboratory product designed to facilitate the detection and analysis of proteins of interest. It is a small, nine-amino acid peptide tag that can be genetically fused to a target protein, allowing for its identification and study using specific anti-Myc antibodies.

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Lab products found in correlation

Ab181591, by Abcam (2 mentions) β actin, by Merck Group (2 mentions) Ab16663, by Abcam (2 mentions) Hpttg, by Thermo Fisher Scientific (2 mentions) P44 42 mapk, by Cell Signaling Technology (2 mentions) Phospho chk1 ser345, by Thermo Fisher Scientific (2 mentions) Mms 101p, by BioLegend (2 mentions) P53 sc 126, by Santa Cruz Biotechnology (2 mentions) Ab17721 and ab90966, by Abcam (2 mentions) Glutathione s transferase beads, by Merck Group (2 mentions) Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Protein g agarose, by Thermo Fisher Scientific (2 mentions) Alexa fluor 594 goat anti mouse antibody, by Thermo Fisher Scientific (2 mentions) Cycloheximide (chx), by Merck Group (2 mentions) Lithium chloride (licl), by Merck Group (2 mentions) Mg132, by Merck Group (2 mentions) Ab4812, by Abcam (2 mentions) Alexa fluor 488 goat anti rabbit antibody, by Thermo Fisher Scientific (2 mentions) α tubulin sc 5286, by Santa Cruz Biotechnology (2 mentions) Ab48031, by Abcam (2 mentions)

Spelling variants of this product

Myc-tag (195 citations) Anti-Myc-Tag (2276), (3 citations) Myc-tag (9B11) mouse mAb (2276S) (2 citations) Monoclonal anti-Myc-Tag (2276) antibodies (2 citations)

5 protocols using myc tag 2276

1

Protein expression analysis by Western blot

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Western blot and immunohistochemical analyses were performed as described previously.20 (link),49 (link) Primary antibodies against PBF (custom20 (link),49 (link)), HA (MMS-101P; BioLegend, San Diego, CA, USA), hPTTG (700791; Invitrogen, Paisley, UK), phospho-p44/42 MAPK (Erk1/2) (9101), p44/42 MAPK (Erk1/2) (4695), Myc-Tag (2276; Cell Signaling Technology, Danvars, MA, USA), p53 (sc-126; Santa Cruz Biotechnology, Dallas, TX, USA), cyclin D1 (ab16663), cyclin A2 (ab181591; Abcam, Cambridge, UK), Chk1 (GTX50463), Brca1 (GTX50557; GeneTex, Irvine, CA, USA), phospho-CHK1(Ser345) (PA5-34625; Thermofisher Scientific, Waltham, MA, USA) and β-actin (A5441; Sigma-Aldrich, Dorset, UK) were used. Densitometry was performed on blots scanned into Photoshop (Adobe Systems) and analysed using ImageJ as described.27 (link)
Read M.L., Fong J.C., Modasia B., Fletcher A., Imruetaicharoenchoke W., Thompson R., Nieto H., Reynolds J.J., Bacon A., Mallick U., Hackshaw A., Watkinson J.C., Boelaert K., Turnell A.S., Smith V.E, & McCabe C.J. (2017). Elevated PTTG and PBF predicts poor patient outcome and modulates DNA damage response genes in thyroid cancer. Oncogene, 36(37), 5296-5308.
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2

Protein expression analysis by Western blot

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blot and immunohistochemical analyses were performed as described previously.20 (link),49 (link) Primary antibodies against PBF (custom20 (link),49 (link)), HA (MMS-101P; BioLegend, San Diego, CA, USA), hPTTG (700791; Invitrogen, Paisley, UK), phospho-p44/42 MAPK (Erk1/2) (9101), p44/42 MAPK (Erk1/2) (4695), Myc-Tag (2276; Cell Signaling Technology, Danvars, MA, USA), p53 (sc-126; Santa Cruz Biotechnology, Dallas, TX, USA), cyclin D1 (ab16663), cyclin A2 (ab181591; Abcam, Cambridge, UK), Chk1 (GTX50463), Brca1 (GTX50557; GeneTex, Irvine, CA, USA), phospho-CHK1(Ser345) (PA5-34625; Thermofisher Scientific, Waltham, MA, USA) and β-actin (A5441; Sigma-Aldrich, Dorset, UK) were used. Densitometry was performed on blots scanned into Photoshop (Adobe Systems) and analysed using ImageJ as described.27 (link)
Read M.L., Fong J.C., Modasia B., Fletcher A., Imruetaicharoenchoke W., Thompson R., Nieto H., Reynolds J.J., Bacon A., Mallick U., Hackshaw A., Watkinson J.C., Boelaert K., Turnell A.S., Smith V.E, & McCabe C.J. (2017). Elevated PTTG and PBF predicts poor patient outcome and modulates DNA damage response genes in thyroid cancer. Oncogene, 36(37), 5296-5308.
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3

Antibody-Based Protein Detection Assay

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Antibodies against human KDM1A (ab17721 and ab90966), CK1δ (ab48031), GATA6 (ab22600), and USP22 (ab4812) were from Abcam. Antibodies against GST (sc-138), Tuj-1(sc-58888), CK1α (sc-6477), CK1ε (sc-6471), BMP2 (sc-6895), CDKN1A (sc-397), and α-Tubulin (sc-5286) were from Santa Cruz Biotechnology. Antibodies against human GSK3β (9315), GSK3α (9338), CD133 (3663), phospho-GSK3β-S9 (9336), Oct4 (2750), H3K4me2/3 (9725 and 9751) and Myc-tag (2276) were from Cell Signaling Technology. Antibodies against phospho-serine/threonine (612548) and nestin (611658) were from BD Transduction Laboratories. Antibodies against hemagglutinin (HA)-Tag and Flag-tag were from Sigma. Hoechst 33342, Alexa Fluor 488 goat anti-rabbit antibody, and Alexa Fluor 594 goat anti-mouse antibody were from Molecular Probes. The specific antibody against phospho-KDM1A S683 was produced by Signalway Antibody. Supplementary Table 1 contains detailed information about the used antibodies.
Temozolomide (TMZ), tideglusib, lithium chloride, glutathione S-transferase (GST) beads, cycloheximide (CHX), and MG132 were from Sigma. Puromycin, hygromycin, and D4476 (4-[4-(2,3-dihydro-benzo[1,4]dioxin-6-yl)-5-pyridin-2-yl-1H-imidazol-2-yl] benzamide) were from EMD Biosciences. Protein G agarose was from Life Technologies. The recombinant active GSK3β (G09-10G) and CK1α (C64-10G) proteins were from SignalChem Lifesciences Corp.
Zhou A., Lin K., Zhang S., Chen Y., Zhang N., Xue J., Wang Z., Aldape K.D., Xie K., Woodgett J.R, & Huang S. (2016). Nuclear GSK3β promotes tumorigenesis by phosphorylating KDM1A and inducing its deubiquitination by USP22. Nature cell biology, 18(9), 954-966.
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4

Antibody-Based Protein Detection Assay

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Antibodies against human KDM1A (ab17721 and ab90966), CK1δ (ab48031), GATA6 (ab22600), and USP22 (ab4812) were from Abcam. Antibodies against GST (sc-138), Tuj-1(sc-58888), CK1α (sc-6477), CK1ε (sc-6471), BMP2 (sc-6895), CDKN1A (sc-397), and α-Tubulin (sc-5286) were from Santa Cruz Biotechnology. Antibodies against human GSK3β (9315), GSK3α (9338), CD133 (3663), phospho-GSK3β-S9 (9336), Oct4 (2750), H3K4me2/3 (9725 and 9751) and Myc-tag (2276) were from Cell Signaling Technology. Antibodies against phospho-serine/threonine (612548) and nestin (611658) were from BD Transduction Laboratories. Antibodies against hemagglutinin (HA)-Tag and Flag-tag were from Sigma. Hoechst 33342, Alexa Fluor 488 goat anti-rabbit antibody, and Alexa Fluor 594 goat anti-mouse antibody were from Molecular Probes. The specific antibody against phospho-KDM1A S683 was produced by Signalway Antibody. Supplementary Table 1 contains detailed information about the used antibodies.
Temozolomide (TMZ), tideglusib, lithium chloride, glutathione S-transferase (GST) beads, cycloheximide (CHX), and MG132 were from Sigma. Puromycin, hygromycin, and D4476 (4-[4-(2,3-dihydro-benzo[1,4]dioxin-6-yl)-5-pyridin-2-yl-1H-imidazol-2-yl] benzamide) were from EMD Biosciences. Protein G agarose was from Life Technologies. The recombinant active GSK3β (G09-10G) and CK1α (C64-10G) proteins were from SignalChem Lifesciences Corp.
Zhou A., Lin K., Zhang S., Chen Y., Zhang N., Xue J., Wang Z., Aldape K.D., Xie K., Woodgett J.R, & Huang S. (2016). Nuclear GSK3β promotes tumorigenesis by phosphorylating KDM1A and inducing its deubiquitination by USP22. Nature cell biology, 18(9), 954-966.
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5

Artesunate and Antibody Experimental Protocol

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Artesunate (ART), purchased from Xi'an HaoYuan Bio Technology Co., Ltd., had a purity of 99.86% and was dissolved in ddH2O for this study.
Antibodies against GAPDH (#5174), P53 (#2524), Flag‐tag (#14793) and myc tag (#2276) were purchased from Cell Signaling Technology, Inc. Antibodies against HMGCR (HPA008338) and LDHA (SAB2108638) were obtained from Sigma‐Aldrich, Inc. Antibodies for ENO1 (ab155102), HK2 (ab104836) and SREBP2 (ab30682) and all secondary antibodies (anti‐mouse, anti‐goat and anti‐rabbit immunoglobulin G) were purchased from Abcam.
Wei S., Liu L., Chen Z., Yin W., Liu Y., Ouyang Q., Zeng F., Nie Y, & Chen T. (2019). Artesunate inhibits the mevalonate pathway and promotes glioma cell senescence. Journal of Cellular and Molecular Medicine, 24(1), 276-284.
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