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609 protocols using smz1500

1

Stereoscopic Imaging of Embryos on Microfluidic Chips

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General stereoscopic images of embryos grown on chip-based devices were obtained using the Leica MZ7.5 stereomicroscope from Leica Microsystems, Wetzlar, Germany. Stereoscopic fluorescence images were taken using a Nikon SMZ1500 from Nikon, Japan. Stereoscopic images were collected with a Nikon SMZ1500 and Nikon E5400 Coolpix camera from Nikon, Japan. Data analysis of collected images was completed using the Leica Application Suite (LAS) (Leica Microsystems) and ImageJ.
For images of Tg (fli1a:EGFP) zebrafish, a Nikon SMZ1500 (Nikon, Tokyo, Japan) connected to a DS-U2/L2 camera was used. Fluorescent images were also taken using an AM4113T-GFBW Dino-Lite Premier USB microscope. Imaging of printed devices was captured using a Leica MZ7.5 stereomicroscope equipped with a Leica DFC295 CMOS camera running under the LAS Multitime software (Leica Microsystems, Wetzlar, Germany). For biocompatibility experiments, the zebrafish were imaged using a Nikon SMZ1500 (Nikon, Tokyo, Japan) with a Nikon E5400 Coolpix camera.
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2

Quantitative Imaging of Embryonic Angiogenesis

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Imaging of embryos was performed using a Nikon SMZ1500 fluorescent stereomicroscope with a Nikon DS-5 digital camera or using a Zeiss Axiophot epifluorescent microscope with a Nikon DXM1200 camera. Images of HUVEC cultures were captured on a Nikon Eclipse TS100 microscope fitted with a DS-Fi1c camera with NIS-Elements D software and images of retinal explants were captured using a Nikon SMZ1500 microscope and DXM1200 camera with ACT-1 software. Data were analysed using Adobe Photoshop and Image J. Quantification of embryonic intersomitic vessel outgrowth and nerve outgrowth between treated and control conditions was measured using Image J. To take into account differences in embryo length results are displayed as a ratio between nerve or vessel length to body or somite length respectively. Statistical analyses were conducted using Prism 5.0 (GraphPad Software, La Jolla, CA) or SPSS 20.0 (SPSS Inc., Chicago, USA). Statistical significance was assessed using two-tailed unpaired Student’s t-test, Mann-Whitney U-test, One-way ANOVA or Kruskal-Wallis test followed by Tukey’s or Dunn’s test respectively. Error bars represent standard error of the mean.
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3

Microscopy Imaging and Visualization

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Whole‐mount and section images were captured using a Nikon SMZ1500 microscope with Nikon DS‐L1 camera. Whole‐mount antibody stains were imaged using a Nikon SMZ1500 and a Nikon DXM1200 camera. Figures were prepared using Adobe Photoshop.
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4

Alleculinae Beetle Specimen Analysis

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The male holotype is deposited at the Museum of Hebei University, Baoding, China (MHBU). The specimen was studied using a Nikon SMZ1500, and the images were taken using a Canon EOS 5D Mark III (Canon Inc., Tokyo, Japan) with a Laowa FF 100 mm F2.8 CA-Dreamer Macro 2× or Laowa FF 25 mm F2.8 Ultra Macro 2.5–5× (Anhui Changgeng Optics Technology Co., Ltd, Hefei, China). The figure of the antenna was drawn by hand using a Nikon SMZ1500 with a camera lucida. Label data are presented verbatim. Line breaks on labels are denoted by a double slash (//); metadata and notes (not written on the labels, themselves) are presented in square brackets ([]). Scientific names are uniformly presented in italics.
Most of the terms in the description are from previous literature (e.g. Young 1975 (link)). The ocular index (OI) was first used to quantify the relative distance between the compound eyes of Alleculinae Laporte, 1840 (Campbell and Marshall 1964 ).
OI=Minimum dorsal distance between compound eyes Maximal dorsal width across compound eyes ×100.
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5

Whole-mount Alizarin Red Staining of Adult Zebrafish Bone

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Whole-mount in-situ Alizarin red staining method was used for staining the bone in the adult zebrafish at 50dpf as described earlier [63 ]. Images were taken after zebrafish were exposed to lethal dose of Tricaine mesylate and then positioned on a 2% agarose plate. Nikon SMZ1500 stereomicroscope and a Nikon D5200 digital camera were used for imaging. Images were edited using Image Composite Editor from Microsoft. Photoshop CS6 was used to improve image quality.
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6

Taxonomy of Parasitoid Wasp Specimen

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The sample was borrowed from the Korean National Arboretum. It was obtained with a Malaise trap and collected in South Korea. It was stored in 95% ethyl alcohol at -19℃. The identity and morphological characters of the specimen were compared with those described in Starý and Schlinger (1967) (link), Starý and González (1978) (link), Bhagat (1981) , van Achterberg (1989) , Jorge Tizado (1992) , Pike et al. (2000) , Tomanović et al. (2002) (link), Čkrkić et al. (2019) (link) and Čkrkić et al. 2020 (link). We used a dissecting microscope (OLYMPUS SZX16, Leica M205C, NIKON SMZ 1500), followed by a DNA extraction.
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7

Bonding Strategies for Pediatric Molars

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Fifteen primary molars were collected and cut as described in the previous section, and all samples were randomly assigned into six groups (n=10 for each group). The samples were randomly grouped by the etching time (0, 15, and 30 s) and adhesive agent (SB2/Single Bond Universal (SBU), 3 M/ESPE, St. Paul, MN, USA) as follows: control, SBU, 35% H3PO4 15 s + SB2, 35% H3PO4 15 s + SBU, 35% H3PO4 30 s + SB2, and 35% H3PO4 30 s + SBU. The samples were embedded and restored with resin for the SBS test. After the SBS test, the fractured test specimen surfaces were examined visually under a stereoscopic loupe (SMZ1500, Nikon, Kanagawa, Japan) at 40× magnification. The fracture types were classified as follows: adhesive failure (failure between the adhesive and enamel), cohesive failure in the enamel, cohesive failure in the resin, and mixed failure (adhesive and cohesive failure in the resin).
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8

Immunostaining and Microscopy of Wing Discs and Notum

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Preparation of wing discs for immunostaining and adult notum for light microscope was performed as described previously30 (link). Confocal imaging was performed using Leica SP2, SP8 and Zeiss LSM 880 microscopy. Primary antibodies used were anti-Sens (guinea pig, a gift from H. Bellen); GFP (rat, NACALAI TESQUE# GF090R). Photograph of adult notum was carried out using Leica MZFLIII microscope and Nikon SMZ1500 microscope.
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9

Stereomicroscopic Imaging of Adult Heads

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Adult heads were mounted on steel pins, immersed in mineral oil, and imaged using a Sony digital camera mounted on a Nikon SMZ1500 stereomicroscope.
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10

Cellophane Overlay Imaging Protocol

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Following protoplast recovery, cellophane overlays were transferred to BCD plates. Same plants were imaged every day for five days using a Nikon SMZ1500 dissecting scope for five days and their length was measured using ImageJ.
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