β-Amyrin was obtained from Sigma–Aldrich (USA) and was dissolved in distilled water to a final concentration of 5 μM. E. coli (BW 25113) was obtained from the Coli Genetic Stock Center (USA) and its purity was ≥ 95% (High-performance liquid chromatography [HPLC]). The cells for experiments were cultured overnight in Luria–Bertani medium at 37°C. Then, cells were purified with centrifugation in 12,000 ×g.
β amyrin
Manufactured by Merck Group
Sourced in Germany, United States, China
β-amyrin is a triterpene compound that can be used as a reference standard or analytical tool in pharmaceutical, biochemical, and scientific research applications. It serves as a pure compound for identification, quantification, and method development purposes.
Automatically generated - may contain errors
Lab products found in correlation
α amyrin, by Merck Group (18 mentions)
Lupeol, by Merck Group (13 mentions)
Oleanolic acid, by Merck Group (13 mentions)
β sitosterol, by Merck Group (10 mentions)
Ursolic acid, by Merck Group (10 mentions)
Stigmasterol, by Merck Group (8 mentions)
Maslinic acid, by Merck Group (6 mentions)
Methanol, by Merck Group (6 mentions)
Betulinic acid, by Merck Group (6 mentions)
Acetonitrile, by Merck Group (5 mentions)
Campesterol, by Merck Group (5 mentions)
Uvaol, by Merck Group (5 mentions)
Formic acid, by Merck Group (5 mentions)
Erythrodiol, by Merck Group (4 mentions)
Acetone, by Merck Group (4 mentions)
Quercetin, by Merck Group (4 mentions)
Rutin, by Merck Group (4 mentions)
Corosolic acid, by Merck Group (4 mentions)
Kaempferol, by Merck Group (3 mentions)
Myricetin, by Merck Group (3 mentions)
31 protocols using β amyrin
1
Purification of E. coli BW 25113
2
Analysis of Phytochemical Compounds from Various Sources
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The following substances and solvents were used in the study: procyanidins B1, B2, C1, A1, A2, A4, (+)-catechin, (–)-epicatechin, quercetin, quercitrin (quercetin-3-O-rhamnoside), isoquercitrin (quercetin-3-O-glucoside), avicularin (quercetin-3-O-arabinofuranoside), guaiaverin (quercetin-3-O-arabinopyranoside), rutin (quercetin-3-O-rutinose), reynoutrin (quercetin-3-O-xyloside), kaempferol, nicotiflorin (kaempferol-3-O-rutinoside), afzelin (kaempferol-3-O-rhamnoside), astragalin (kaempferol-3-O-glucoside), chlorogenic acid (3-O-caffeoylquinic acid), neochlorogenic acid (5-O-caffeoylquinic acid), cryptochlorogenic acid (4-O-caffeoylquinic acid), p-coumaric acid, arbutin, hydroquinone, α-amyrin, β-amyrin, β-sitosterol, lupeol, erythrodiol, maslinic acid, oleanolic acid, acetonitrile, acetone, and methanol, which were purchased from Sigma–Aldrich (Steinheim, Germany); hyperoside (quercetin-3-O-galactoside), procyanidin B3, uvaol, friedelin, 6″-O-acetylisoquercitrin (quercetin-3-O-(6″-acetylglucoside)), betulin, and betulinic and corosolic acids from Extrasynthese (Genay, France); ursolic acid from Carl Roth (Karlsruhe, Germany); and trifluoroacetic acid from Merck (Darmstadt, Germany). All used chemicals were of HPLC grade. Ultrapure water used in this study was prepared with Milli–Q® 180 (Millipore, Bedford, MA, USA) water purification system.
3
Quantitative Analysis of Triterpenoids
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All reagents were of analytical grade. Limonene, lupenone, β-amyrin and α-amyrin standards were obtained from Sigma-Aldrich (St. Louis, MO, USA), and lupeol from Cayman Chemical (Ann Arbor, MI, USA). Methanol and acetonitrile, both HPLC grade, were purchased from VWR Chemicals (Randnor, PA, USA). Ethyl acetate and chloroform super purity solvent were purchased from Romil (Cambridge, UK), and tetrahydrofuran (GPC grade) and isopropanol (HPLC grade) from Scharlau (Barcelona, Spain). Ultrapure water was obtained from an Adrona system (Riga, Latvia). Water was filtered through 0.22 µm nylon membranes purchased from GVS (Sandfor, ME, USA) before use.
Stock solutions of the analytes (1000 µg mL−1) were prepared by dissolving the appropriate amounts of the commercial standards in methanol. Working solutions of the analytes and their mixtures were prepared by diluting the stock solutions with methanol (unless otherwise stated). All solutions were stored at 4 °C until use.
Stock solutions of the analytes (1000 µg mL−1) were prepared by dissolving the appropriate amounts of the commercial standards in methanol. Working solutions of the analytes and their mixtures were prepared by diluting the stock solutions with methanol (unless otherwise stated). All solutions were stored at 4 °C until use.
4
Analytical Standards for Phytochemical Research
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Analytical grade solvents (acetone, dichloromethane, ethanol, ethyl acetate, hexane and methanol) and HPLC-grade methanol and acetonitrile were purchased from Tedia (Fairfield, USA). Water was processed by Milli-Q filter (Millipore Corporation, Billerica, USA). Phorbol 12-myristate 13-acetate (PMA) and lipopolysaccharide (LPS) were from Sigma-Aldrich (USA), dexamethasone, dimethyl sulfoxide (DMSO) were from Sigma-Aldrich (USA), Merck (USA), Hospira (Australia) and MP Biomedical Inc. (USA) respectively. Chemical standards for artemetin, casticin and vitexilactone were from ChemFaces (China), while α-amyrin, β-amyrin, butylated hydroxytoluene (BHT), campesterol, 2,4-Di-tert-butylphenol, maslinic acid, phytol, β-sitosterol, and stigmasterol were from Sigma-Aldrich (USA).
5
Comprehensive Phytochemical Analysis Protocol
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δ-tocopherol, (+)-γ-tocopherol, (±)-α-tocopherol, D-α-tocotrienol, D-δ-tocotrienol, γ-tocotrienol (analytical standards), methyl palmitate (≥99.0%), methyl stearate (≥99.5%), methyl oleate (≥99.0%), methyl linoleate (≥98.5%), methyl linolenate (≥99.0%), betulinic (≥98%), oleanolic (≥97%), ursolic (≥90%) acids, α-amyrin (≥98%), β-amyrin (≥98.5%), lupeol (≥94%), chlorogenic (≥95%), neochlorogenic (≥98%) acids, kaempferol (≥90%), apigenin (≥97%), myricetin (≥96%), quercetin (≥95%), 2-propanol (99.9%), hexane (≥95%), gradient grade acetonitrile, methanol. and ethanol, trifluoroacetic acid (≥99%), and trimethylsulfonium hydroxide (TMSH) (0.25 M methanolic solution) were purchased from Sigma Aldrich (St. Louis, MO, USA). lupeol acetate (≥98%), α-amyrin acetate (≥98%), and β-amyrin acetate (≥98%) were provided by ALB Technology (ALB Materials Inc, Henderson, NV, USA). Water for HPLC was purified by ULTRAPURE Millipore Direct-Q® 3UV-R (Merck Millipore, Billerica, MA, USA). Tert-butyl methyl ether (TBME) (99.8%) was purchased from Avantor Performance Materials Poland S.A. (Gliwice, Poland).
6
Quantification of Phytochemical Standards
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The commercial standards: rutin, β-carotene, β-amyrin, β-sitosterol, lupeol, ursolic acid and linoleic acid were procured (Sigma Aldrich, USA). The AR grade organic solvents (hexane, ethyl acetate, chloroform and methanol) and p-anisaldehyde were purchased (BDH, UK). HPLC grade methanol (Merck, Germany) was used for the preparation of standard stock solutions (1000 μg/ml) and their serial dilutions (β-amyrin and β-sitosterol: 10–100 μg/ml; lupeol and ursolic acid: 10–120 μg/mL).
7
Identification of Polyphenol Compounds
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All the chemicals used in the experiments were of an analytical grade. Cyanidin chloride, cyanidin-3-galactoside, cyanidin-3-glucoside, cyanidin-3-arabinoside, peonidin chloride, peonidin-3-arabinoside, peonidin-3-glucoside, peonidin-3-galactoside, myricetin-3-galactoside, malvidin-3-galactoside, malvidin chloride, malvidin-3-arabinoside, delphinidin-3-galactoside (Extrasynthese, Genay, France), quercetin-3-O-glucoside (Biochemistry, Buchs, Switzerland), quercetin-3-galactoside, quercetin (Carl Roth, Karlsruhe, Germany), myricetin, quercetin-3-rhamnoside, quercetin-3-α-l -arabinofuranoside, quercetin-3-α-l -arabinopyranoside, oleanolic acid, ursolic acid, α-Amyrin, β-Amyrin, corosolic acid, and maslinic acid (Sigma-Aldrich, Steinheim, Germany) were used as standards for the identification of the compounds. Acetone, methanol, acetonitrile, hydrochloric acid, and 4-(Dimethylamino) cinnamaldehyde) were purchased from Sigma-Aldrich (Steinheim, Germany). Ethanol 96% (v/v) was purchased from AB Stumbras (Kaunas, Lithuania). Formic acid was bought from Merck (Darmstadt, Germany).
8
Phytochemical Analysis of Medicinal Plants
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All solvents, reagents, and standards were of analytical reagent grade. Acetone, methanol, acetonitrile, hydrochloric acid, 4-(Dimethylamino) cinnamaldehyde and reference standarts for β-Amyrin, maslinic acid, oleanolic acid, corosolic acid, ursolic acid, α-Amyrin, quercetin-3-rhamnoside, myricetin, quercetin-3-α-L-arabinopyranoside and quercetin-3-α-L-arabinofuranoside were purchased from Sigma-Aldrich (Steinheim, Germany). Ethanol 96% (v/v) was purchased from AB Stumbras (Kaunas, Lithuania), formic acid was bought from Merck (Darmstadt, Germany), and Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) was purchased from Scharlau (Sentmenat, Spain). Reference standards for myricetin-3-galactoside, cyanidin chloride, peonidin chloride, malvidin-3-galactoside, malvidin-3-arabinoside, malvidin chloride, peonidin-3-arabinoside, cyaniding-3-galactoside, delphinidin-3-galactoside, peonidin-3-glucoside, peonidin-3-galactoside, cyaniding-3-glucoside and cyaniding-3-arabinoside were purchased from Extrasynthese (Genay, France). Standards for quercetin and quercetin-3-galactoside were purchased from Carl Roth (Karlsruhe, Germany). The standard for quercetin-3-O-glucoside was bought from Biochemistry (Buchs, Switzerland). Purified deionized water was provided using a water purification system (Milli-Q®, Millipore, Bedford, MA, USA).
9
Analytical Characterization of Phytochemicals
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Ursolic acid, oleanolic acid, betulinic acid, lupeol, lupeol acetate, β-amyrin, β-amyrin acetate, chlorogenic acid, and protocatechuic acid were purchased from Sigma–Aldrich (St. Louis, MO, United States). Methanol, ethanol, and dimethyl sulfoxide (DMSO) were at least for analysis grade from Polish Reagents (POCH, Gliwice, Poland). HPLC-grade acetonitrile, trifluoroacetic acid, and phosphoric acid were purchased from Merck (Merck, Darmstadt, Germany). Water for HPLC was purified by ultrapure Milipore Direct-Q® 3UV–R (Merck). The analysis was performed on VWR Hitachi Chromaster 600 chromatograph with a PDA detector and EZChrom Elite software (Merck). Mass spectrometry was conducted with the use of mass spectrometer micrOTOF-Q II and data was handled by Compass DataAnalysis software version 4.1 (Bruker Daltonics, Bremen, Germany).
10
Isolation and Purification of Triterpenoids
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α-amyrin and β-amyrin of 98.5% purity were purchased from Sigma-Aldrich (Shanghai, China). Ursolic acid and oleanolic acid of 98% purity were purchased from Energy Chemical (Shanghai, China). Hederagenin of 98% purity was bought from TCI (Shanghai, China). Gypsogenic acid of 95% purity was purchased from TRC (Beijing, China). α-Boswellic acid of 98% purity was purchased from PUSH BIO-TECHNOLOGY (Sichuan, China).
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