Nitric acid (HNO3) and hydrochloric acid (HCl) were purchased from Merck (Darmstadt, Germany). Dimethylarsinic acid (DMA), hydrazine sulfate, hydrobromic acid, and other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA). Rice flour (standard reference material [SRM] 1568a) was obtained from the National Institute of Standards and Technology (Gaithersburg, MD, USA). All standard solutions, reagents, and samples were prepared using deionized water (18 MΩcm). To avoid arsenic residue contamination, all glassware was soaked in 10% (v/v) HNO3 overnight and washed three times with deionized water before use.
Hydrazine sulfate
Manufactured by Merck Group
Sourced in United States
Hydrazine sulfate is a chemical compound used in various laboratory applications. It is a crystalline solid that is soluble in water and other polar solvents. The core function of hydrazine sulfate is as a reducing agent and reagent in chemical reactions and analyses.
Automatically generated - may contain errors
Lab products found in correlation
Hydrobromic acid, by Merck Group (1 mentions)
Nitric acid hno3, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
5 fluorouracil, by Merck Group (1 mentions)
β nicotinamide adenine dinucleotide, by Merck Group (1 mentions)
Bhi broth, by Merck Group (1 mentions)
Glycine, by Merck Group (1 mentions)
Guaiacol, by Merck Group (1 mentions)
L lactate, by Merck Group (1 mentions)
Sodium citrate, by Merck Group (1 mentions)
L lactate oxidase, by Merck Group (1 mentions)
Ammonia solution, by Merck Group (1 mentions)
K 2 4, by Merck Group (1 mentions)
H2ptcl6, by Merck Group (1 mentions)
Haucl4, by Merck Group (1 mentions)
Cacl2, by Merck Group (1 mentions)
Na2hpo4, by Merck Group (1 mentions)
Spectramax plus 384, by Molecular Devices (1 mentions)
Nhs lc lc biotin, by Thermo Fisher Scientific (1 mentions)
Dowex 50wx8, by Merck Group (1 mentions)
7 protocols using hydrazine sulfate
1
Arsenic Determination in Rice Flour
2
Betulin Extraction from Birch Bark
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Betulin was isolated from Betula Pendula bark using the methods in [26 (link)]. 5-fluorouracil (Sigma Aldrich, Moscow, Russia), hydrazine sulfate (Sigma Aldrich), purified water (resistivity ≥18 MΩ·cm, Millipore, Merck, Darmstadt, Germany).
3
Lactic Acid Analysis in Oral Biofilms
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Among the final products of oral microorganisms, lactic acid was analyzed because it is the strongest acid metabolite, presenting a pKa (acid dissociation constant) of 3.810. In the fifth day of the biofilm growth process, the medium was renewed and the plates were incubated for further 3.5 h. Lactic acid analysis was carried out in the culture media (BHI broth).
The amount of lactic acid was determined by the enzymatic method13 . In 96-wells plates, 85 mL of 1 mol/L glycine (Sigma Aldrich, Saint Louis, Missouri, USA), 85 mL of 0.8 mol/L hydrazine sulfate (Sigma Aldrich, Saint Louis, Missouri, USA), 20 mL of 26 mmol/L b-nicotinamide adenine dinucleotide (Sigma Aldrich, Saint Louis, Missouri, USA) and 10 mL of BHI broth or L-lactic acid standards (0.2 to 3.0 mmol/L; Sigma Aldrich, Saint Louis, Missouri, USA) were added. The absorbance was read at 340 nm (A1). Then, 10 mL of 1.0 g/L L-lactic dehydrogenase were added. After 1 h at room temperature, a new reading was performed at 340 nm (A2). The quantity of lactic acid (DL) was obtained by the equation DL=A2-A1. The DL values were converted to mmol/L of lactic acid, according to the readings of L-lactic acid standards.
The amount of lactic acid was determined by the enzymatic method
4
Lactate Biosensor Development Protocol
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Delignified cellulose (Greencel) was obtained from Bukoza and was used without any additional pre-treatment. L-lactate oxidase (EC 1.1.3.2, LOx) from Pediococcus sp. with specific activity ≥ 20 U·mg−1 solid, Nafion®, hemin, guaiacol, hydrazine sulfate, L-lactate, HAuCl4, and H2PtCl6 were obtained from Sigma-Aldrich. In addition, 28% ammonia solution, Na2HPO4, CaCl2, KН2РО4, HNO3, and sodium citrate were purchased from Merck, and 30% H2O2 from Thermo Fisher Scientific. All reagents and solutions were prepared using ultrapure water.
5
Enzymatic Assays for Enolase and Aldolase
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Enolase activity was measured directly by monitoring the formation of Phosphoenol pyruvate (PEP) using SPECTRAmax PLUS 384 (Molecular Devices Corp.) at 240 nm as described earlier [31] (link). The assay was performed at 25°C in a 1.0 mL reaction mixture containing 20 mM Tris–HCl, pH 7.5, 2 mM MgSO4, 75 mM KCl and 1 mM 2-phosphoglycerate (2PGA) or 2 mM PEP. For the determination of the values of kinetic parameters, the substrate concentrations were varied from 0 to 10 mM for 2PGA and 0 to 7.5 mM for PEP.
Aldolase activity was measured by using hydrazine assay modified by Jagannathan et al[32] (link) using D-Fru-1,6-P2 (Sigma) as substrate and hydrazine sulfate (Sigma) as detection reagent for the formed 3-phosphoglyceraldehyde. The assay was performed in a 1.0 mL reaction mixture and the absorbance was recorded at 240 nm. To calculate Vmax and Km, the activity was measured between 0.1 and 7 mM D-Fru-1, 6-P2.
Aldolase activity was measured by using hydrazine assay modified by Jagannathan et al[32] (link) using D-Fru-1,6-P2 (Sigma) as substrate and hydrazine sulfate (Sigma) as detection reagent for the formed 3-phosphoglyceraldehyde. The assay was performed in a 1.0 mL reaction mixture and the absorbance was recorded at 240 nm. To calculate Vmax and Km, the activity was measured between 0.1 and 7 mM D-Fru-1, 6-P2.
6
PF4 Interaction with Heparin and Enoxaparin
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HP and LMWH (enoxaparin) were obtained from the United States Pharmacopoeia convention. Recombinant human PF4 was purchased from Sino Biological (Beijing, China). NHS-LC-LC-Biotin was purchased from Thermo Fisher Scientific (Shanghai, China). Deuterium oxide (99.9% D2O) was obtained from Qingdao Tenglong Weibo Technology Co., Ltd. (Qingdao, China). The 70% aqueous hydrazine was obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Hydrazine sulfate, dimethyl sulfoxide, pyridine and Dowex 50WX8 (hydrogen form, 50 to 100 mesh) were purchased from Sigma-Aldrich (Shanghai, China). N, O-bis(trimethylsilyl)acetamide (BTSA) was obtained from Jinming Biotechnology (Beijing, China). Phosphate-Buffered Saline (PBS) was purchased from Wuhan Procell Biological Technology (Wuhan, China).
7
Cytotoxicity Evaluation of Metronidazole
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3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Sigma, Saint Louis, USA), hydrazine sulfate, ammonium heptamolybdate tetrahydrate, ammonium acetate, acetic acid, tetrabutylammonium bromide (TBAB), cetyltrimethylammonium bromide (CTAB), methyl methacrylate (MMA), benzoyl peroxide (BPO), and all of the used solvents were purchased from Sigma-Aldrich or some of the other chemical companies. All of the other chemicals were analytical grade and applied without further purification. Metronidazole (MTN) was purchased from Amin pharmacy (Iran).
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