Enzychrom alanine transaminase assay kit

Serum levels of inflammatory and liver damage markers were quantified with the following commercially available reagents: mouse TNF-α Quantikine ELISA Kit (R&D Systems, Minneapolis, MN, United States), IL-6 Mouse ELISA Kit (Thermo Scientific, Rockford, IL, United States), IL-10 Mouse ELISA Kit (Thermo Scientific), and EnzyChrom^TM Alanine Transaminase Assay Kit (BioAssay Systems, Hayward, CA, United States).

Serum samples were collected using orbital venous plexus bleeding in anesthetized mice. Alanine transaminase (ALT) and alkaline phosphatase (ALP) activities were determined using an EnzyChromTM Alanine Transaminase Assay Kit (BioAssay Systems) and a QuantiChromTM Alkaline Phosphatase Assay Kit (BioAssay Systems) [14 (link), 15 (link)], respectively.

Serum samples were collected from eyeball blood in anesthetized mice. Alanine transaminase (ALT) and alkaline phosphatase (ALP) activities were determined using an EnzyChrom^TM Alanine Transaminase Assay Kit and QuantiChrom^TM Alkaline Phosphatase Assay Kit (BioAssay Systems), respectively.

Hepatic injury was assessed by serum alanine transaminase (ALT) levels with use of the EnzyChrom Alanine Transaminase Assay Kit (BioAssay Systems, Hayward, CA).

To determine the serum levels of interferon-γ, TNF-α, ASAT and ALAT, mouse IFN-γ ELISA kit (Pierce Biotechnology, Rockford, IL, USA), mouse TNF-alpha Quantikine ELISA kit (R&D Systems, MA, USA), EnzyChrom™ Aspartate Transaminase Assay Kit or EnzyChrom™ Alanine Transaminase Assay Kit (both BioAssay Systems, Hayward, CA, USA), respectively, were used according to the manufacturer’s instructions.

Liver alanine transaminase (ALT) activities were determined using EnzyChrom Alanine Transaminase Assay Kit (BioAssay Systems) according to the manufacturer’s instructions. For histopathological examination, the liver was taken from mice on day 10 after infection and fixed with 10% formalin in PBS. Sections were stained with Hematoxylin and Eosin.

To determine the serum levels of IFN gamma, TNF alpha, ASAT, ALAT, and NO, a mouse IFN gamma ELISA kit (Pierce Biotechnology, Rockford, IL, USA), a mouse TNF-alpha Quantikine ELISA kit (R&D Systems, MA, USA), the EnzyChrom™ Aspartate Transaminase Assay Kit, the EnzyChrom™ Alanine Transaminase Assay Kit (both BioAssay Systems, Hayward, CA, USA) and the Nitrate/Nitrite Colorimetric Assay Kit (Cayman Chemical Company, Michigan, USA), respectively, were used according to the manufacturer instructions. Creatinine was determined by means of the Jaffé reaction. Briefly, in a strongly alkaline medium, picric acid is added to the sample. Under these conditions, it reacts with creatinine to form an orange-red complex, which can be measured photometrically at 492 nm. Serum urea was measured using the commercially available colorimetric Urea Assay Kit (Sigma-Aldrich Chemie GmbH, Steinheim, Germany), which utilizes coupled enzyme reactions involving urease and glutamate dehydrogenase, resulting in a product that can be detected at 570 nm.