Mouse monoclonal anti ph2ax ser 139

To evaluate protein expression, the following antibodies were used: mouse monoclonal anti-pH2AX (Ser 139) (1:100) (Santa Cruz Biotechnology Inc., Dallas, TX, USA, sc-517348), rabbit monoclonal anti-Vimentin (1:500) (Cell Signaling, Danvers, MA, USA, 5741), rabbit monoclonal anti-PTEN (1:500) (Cell Signaling, Danvers, MA, USA, 9559), mouse monoclonal anti-p53 (1:100) (clone DO-1, Santa Cruz Biotechnology Inc., Dallas, TX, USA, sc-126), rabbit polyclonal anti-cMyc (1:1000) (Proteintech, Rosemont, IL, USA, 10828-1-AP), and rabbit monoclonal anti-Bcl-xL (1:500) (Cell Signaling, Danvers, MA, USA, 2764). Mouse monoclonal anti-β-actin (1:10,000) (Sigma Aldrich, A2228) was used as loading control. Goat anti-mouse IgG-HRP (1:10,000) (Bethyl Laboratories, Montgomery, TX, USA, A90-116P) and goat anti-rabbit IgG-HRP (1:10,000) (Bethyl Laboratories, Montgomery, TX, USA, A120-101P) were used as secondary antibodies.

To evaluate protein expression on Western blot membranes the following antibodies were used: mouse monoclonal anti-pH2AX (Ser 139) (1:100) (Santa Cruz Biotechnology Inc., Dallas, TX, USA, sc-517348), mouse monoclonal anti-CHK1 (1:100) (clone F-11, Santa Cruz Biotechnology Inc., Dallas, TX, USA, sc-8408), mouse monoclonal anti-RAD51 (1:100) (clone G-4, Santa Cruz Biotechnology Inc., Dallas, TX, USA, sc-398587), mouse monoclonal anti-p53 (1:100) (clone DO-1, Santa Cruz Biotechnology Inc., Dallas, TX, USA, sc-126), rabbit polyclonal anti-p21 (1:200) (clone C-19, Santa Cruz Biotechnology Inc., Dallas, TX, USA, sc-397), rabbit polyclonal anti-HSP70 (1:1000) (Proteintech, Rosemont, IL, USA, 10995-1-AP) and rabbit polyclonal anti-HSP90 (1:1000) (Proteintech, Rosemont, IL, USA, 13171-1-AP). Mouse monoclonal anti-β-actin (1:10,000) (Sigma Aldrich, Burlington, MA, USA) or rabbit polyclonal anti-Histone H3 (4499, Cell Signaling, Danvers, MA, USA) were used as loading control. The goat anti-mouse IgG-HRP (1:30,000) (Bethyl Laboratories, Montgomery, TX, USA, A90-116P) and goat anti-rabbit IgG-HRP (1:30,000) (Bethyl Laboratories, Montgomery, TX, USA, A120-101P) were used as secondary antibodies. All the primary and secondary antibodies were diluted in PBS-0.1% Tween20 solution containing 3% of BSA (SERVA).