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Sa alkaline phosphatase

Manufactured by Vector Laboratories
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SA-alkaline phosphatase is a laboratory reagent used for the detection and quantification of alkaline phosphatase activity in various biological samples. It is a conjugate of streptavidin and alkaline phosphatase, which binds to biotinylated molecules and catalyzes a colorimetric or chemiluminescent reaction, allowing the visualization and measurement of target analytes.

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Lab products found in correlation

Histone, by Merck Group (2 mentions) Elisa plate, by Thermo Fisher Scientific (2 mentions) Anti igm or anti igg, by Thermo Fisher Scientific (2 mentions) Biotinylated anti mouse igm, by Jackson ImmunoResearch (2 mentions) Alkaline phosphatase conjugated anti mouse igg, by Thermo Fisher Scientific (2 mentions) Biotinylated anti kappa ab, by Thermo Fisher Scientific (2 mentions) Salmon sperm dsdna, by Thermo Fisher Scientific (2 mentions)

2 protocols using sa alkaline phosphatase

1

ELISA-based Antibody Titer Measurement

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Ig and ANA titers were measured by ELISA as previously described (Wong et al., 2012 (link)). Briefly, for IgG or IgM detection, ELISA plates (Thermo Fisher Scientific, Waltham, MA) were coated with anti-IgM or anti-IgG (Invitrogen, Grand Island, NY) capture antibodies and detected using biotinylated antimouse IgM (Jackson ImmunoResearch, West Grove, PA) or alkaline-phosphatase conjugated anti-mouse IgG (Molecular Probes, Grand Island, NY). Total IgG autoAb titers were measured in plates coated with salmon sperm dsDNA (Invitrogen, Grand Island, NY), histone (Sigma Aldrich, St. Louis, MO), or nucleosome and detected with biotinylated anti-kappa Ab (Invitrogen, Grand Island, NY). IgG subtype-specific autoAb titers were detected by biotinylated-IgG1, -IgG2b, and AP-IgG2c Abs (Southern Biotech, Birmingham, AL). Biotinylated antibodies were detected by SA-alkaline phosphatase (Vector Laboratories, Burlingame, CA). The plates were developed by the PNPP (p-nitrophenyl phosphate, disodium salt; Thermo Fisher Scientific, Rockford, IL) substrates for alkaline phosphatase and read at γ405 nm.
Domeier P.P., Chodisetti S.B., Schell S.L., Kawasawa Y.I., Fasnacht M.J., Soni C, & Rahman Z.S. (2018). B-Cell-Intrinsic Type 1 Interferon Signaling Is Crucial for Loss of Tolerance and the Development of Autoreactive B Cells. Cell reports, 24(2), 406-418.
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2

ELISA-based Antibody Titer Measurement

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Ig and ANA titers were measured by ELISA as previously described (Wong et al., 2012 (link)). Briefly, for IgG or IgM detection, ELISA plates (Thermo Fisher Scientific, Waltham, MA) were coated with anti-IgM or anti-IgG (Invitrogen, Grand Island, NY) capture antibodies and detected using biotinylated antimouse IgM (Jackson ImmunoResearch, West Grove, PA) or alkaline-phosphatase conjugated anti-mouse IgG (Molecular Probes, Grand Island, NY). Total IgG autoAb titers were measured in plates coated with salmon sperm dsDNA (Invitrogen, Grand Island, NY), histone (Sigma Aldrich, St. Louis, MO), or nucleosome and detected with biotinylated anti-kappa Ab (Invitrogen, Grand Island, NY). IgG subtype-specific autoAb titers were detected by biotinylated-IgG1, -IgG2b, and AP-IgG2c Abs (Southern Biotech, Birmingham, AL). Biotinylated antibodies were detected by SA-alkaline phosphatase (Vector Laboratories, Burlingame, CA). The plates were developed by the PNPP (p-nitrophenyl phosphate, disodium salt; Thermo Fisher Scientific, Rockford, IL) substrates for alkaline phosphatase and read at γ405 nm.
Domeier P.P., Chodisetti S.B., Schell S.L., Kawasawa Y.I., Fasnacht M.J., Soni C, & Rahman Z.S. (2018). B-Cell-Intrinsic Type 1 Interferon Signaling Is Crucial for Loss of Tolerance and the Development of Autoreactive B Cells. Cell reports, 24(2), 406-418.
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