Six strains of MRSA were isolated from contact lenses, kohl samples, and patients with corneal infection. Identification and characterization of S aureus were carried out using morphological microscopic study, coagulase, and catalase tests.13 (link) All the bacterial strains were examined for methicillin resistance. The disk-diffusion assay14 (link) was utilized with a 1 µg oxacillin disk (Oxoid). The sizes of IZ were recorded after 24 hours at 35°C. Strains with IZ ≤8 mm were considered methicillin resistant.15 The various strains of S aureus were also identified by analysis of 16S ribosomal RNA (16S rRNA) genes for bacterial species.16
Oxacillin disk
Manufactured by Thermo Fisher Scientific
The Oxacillin disk is a laboratory diagnostic device used to determine the susceptibility of bacteria to the antibiotic oxacillin. It is used as part of the antimicrobial susceptibility testing process to help guide appropriate antibiotic treatment decisions.
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Lab products found in correlation
2 protocols using oxacillin disk
1
Characterization of MRSA from Clinical Samples
2
Detecting Methicillin Resistance in S. aureus
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S. aureus isolates were tested for methicillin resistance using the cefoxitin and oxacillin disk diffusion methods outlined by the Clinical and Laboratory Standards Institute [32] . Cefoxitin disk (30 µg, Oxoid) and oxacillin disk (1 µg, Oxoid) were used in this study. The zones of inhibition were measured after 18–20 hours incubation at 35°C. Isolates with zone sizes less than 21 mm for cefoxitin and 10 mm for oxacillin were considered methicillin resistant according to the criteria of CLSI [32] .
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S. aureus isolates were tested for methicillin resistance using the cefoxitin and oxacillin disk diffusion methods outlined by the Clinical and Laboratory Standards Institute [32] . Cefoxitin disk (30 µg, Oxoid) and oxacillin disk (1 µg, Oxoid) were used in this study. The zones of inhibition were measured after 18–20 hours incubation at 35°C. Isolates with zone sizes less than 21 mm for cefoxitin and 10 mm for oxacillin were considered methicillin resistant according to the criteria of CLSI [32] .
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