A2780 cells were seeded into 6-well plates and cultured overnight. After 24 h treatment, cells were trypsinized, washed with PBS and harvested by centrifugation. Apoptotic cells were detected using an Annexin V-FITC/PI double staining apoptosis detection kit (Beyotime Biotechnology, Shanghai, China). Briefly, a total of 10,000 cells were collected and analyzed by BD FACS Canto™ flow cytometry (BD Biosciences, San Jose, CA, USA).
Annexin 5 fitc pi double staining apoptosis detection kit
Manufactured by Beyotime
Sourced in China
The Annexin V-FITC/PI double staining apoptosis detection kit is a laboratory product used to identify and quantify apoptosis, a form of programmed cell death. The kit utilizes Annexin V, a calcium-dependent phospholipid-binding protein, and propidium iodide (PI), a DNA-binding dye, to differentiate between viable, early apoptotic, and late apoptotic/necrotic cells.
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Lab products found in correlation
Facscanto, by BD (1 mentions)
Shp 2, by Proteintech (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Fatal bovine serum, by Thermo Fisher Scientific (1 mentions)
Doxorubicin, by Beyotime (1 mentions)
β actin, by Proteintech (1 mentions)
Crystal violet, by Beyotime (1 mentions)
Bcl2 associated x (bax), by Proteintech (1 mentions)
Fluo 3 am, by Beyotime (1 mentions)
Bcl 2, by Proteintech (1 mentions)
Reactive oxygen species assay kit, by Beyotime (1 mentions)
Mmp assay kit, by Beyotime (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide, by Beyotime (1 mentions)
Penicillin streptomycin antibiotics, by Solarbio (1 mentions)
2 protocols using annexin 5 fitc pi double staining apoptosis detection kit
1
Annexin V-FITC/PI Apoptosis Assay
2
Apoptosis and ROS Regulation in Cancer Cells
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PSN-A (Figure 1 A) was purchased from EXTRASYNTHESE (Genay, France). Dulbecco's Modified Eagle's Medium (DMEM) and fatal bovine serum (FBS) were purchased from Gibco (Eggenstein, Germany) while Penicillin/ Streptomycin antibiotics was obtained from Solarbio co., Ltd. (Beijing, China). Annexin V-FITC/PI double staining apoptosis detection kit, Reactive Oxygen Species (ROS) assay kit, mitochondrial membrane potential (MMP) assay kit, Crystal violet, 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT), dimethyl sulfoxide (DMSO), Fluo-3AM and doxorubicin were purchased from Beyotime Institute of Biotechnology (Haimen, Jiangsu, China). The primary antibodies for cleaved caspases-3, cleaved PARP, p-STAT3 (Tyr705), STAT3 and SHP-2 were purchased from Cell Signaling Technology (Beverly, MA) while primary antibodies for Bax, Bcl-2, SHP-2, PTEN and β-actin were purchased from Proteintech (Wuhan, China). The primary antibodies for p-JAK2 and JAK2 were purchased from abcam (Cambridge, MA). Horseradish peroxidase (HRP)-conjugated secondary antibodies (goat anti-rabbit, goat anti-mouse) were obtained from Sigma.
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