Uas puc

All the fly stocks were raised on standard Drosophila corn media and crosses were performed at 25°C unless otherwise indicated. UAS-APLP2 was kindly provided by Dr. Merders; puc^H246, UAS-puc-IR, UAS-mmp1-IR, and UAS-p35 were obtained from Bloomington Stock Center; UAS-Bsk^DN, UAS-puc[26 (link)], ptc-Gal4, en-Gal4, puc^E69[29 (link)], and UAS-LacZ [30 (link)] were previously described.

The following stocks were used: UAS-Ras^V12 (Lee et al., 1996 (link)); nub-Gal4 (Calleja et al., 1996 (link)); hid 5′F-GFP (Tanaka-Matakatsu et al., 2009 (link)); UAS-DER^DN, UAS-Diap1, UAS-puc, ap-Gal4, puc-LacZ, rpr-LacZ, and brk-LacZ (Bloomington Drosophila Stock Center); mys^RNAi, UAS-diβ (Martin-Bermudo and Brown, 1999 (link)) and hid^RNAi (Vienna Drosophila RNA-i Center). To generate mutant clones in the wing disc we used the FRT/FLP technique (Chou and Perrimon, 1992 (link)). Mutant clones were marked by the absence of GFP. The following mutant alleles and chromosomes were used: mys¹¹ [also known as mys^XG43 (Bunch et al., 1992 (link))] and e22c-Gal4 UAS-flipase (Duffy et al., 1998 (link)). The e22c-Gal4 driver is expressed in some posterior wing disc cells and was therefore used in combination with UAS- flp to generate large mys clones. Flies were raised at 25°C.

All stocks were raised on standard Drosophila media and crosses were performed at 25°C unless otherwise indicated. UAS-APLP1, UAS-APPL^sd were kindly provided by Dr. Merdes [22 (link)]; puc^H246, UAS-mmp1-IR, UAS-mmp2-IR, UAS-TIMP1, UAS-Ras^V12, UAS-Rho1-IR, UAS-p35, UAs-P53^DN were obtained from Bloomington Stock Center; sd-Gal4, UAS-Bsk^DN, UAS-Puc, UAS-Egr, UAS-Hep [70 (link)]; ptc-Gal4, en-Gal4, puc^{E69 34}, UAS-LacZ [71 (link)] were previously described.