Cd16 56

Manufactured by BD

Sourced in United States

CD16/56 is a lab equipment product that is used for the detection and analysis of specific cell surface markers. It is designed to identify and quantify natural killer cells and natural killer T cells in biological samples.

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Lab products found in correlation

Facscalibur, by BD (3 mentions) 70 mm cell strainer, by BD (1 mentions) Cd19 apc, by BD (1 mentions) Cellquest software, by BD (1 mentions) Hla dr, by BD (1 mentions) Cd103, by BD (1 mentions) Attune nxt, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

CD16,56-PE (4 citations) Anti-CD16/56 PE (3 citations)

6 protocols using cd16 56

Bronchoalveolar Lavage Cytology and Flow Cytometry

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BAL differential cell count and BAL TBNK immunophenotyping were performed at diagnosis, as previously described [14 (link),18 (link)]. In brief: we instilled seven aliquots of 20 ml and one of 10 ml, up to a total volume of 150 ml of saline. After each instillation, the aliquot was immediately recovered, pooled, and thereafter processed for cytological and flow cytometry analysis. Cytospin preparations were stained according to the May–Grünwald–Giemsa and Papanicolaou methods and differential cell count was performed by counting 200 cells.
For TBNK immunophenotyping, the BALF was strained through a 70 mm cell strainer (BD Biosciences), centrifuged and resuspended in Haemaccel (Behringwerke, Marburg, Germany), and incubated with the respective mAbs (CD3, CD4, CD8, CD14, CD19, CD45, CD16/56, BD Biosciences, San Jose, CA, USA) for 15 min, followed by lysing, washing, and fixation. Labeled cells were analyzed by FACSCalibur and SimulSET software (BD Biosciences).

Osolnik K., Rijavec M, & Korosec P. (2014). Disposal of iNKT cell deficiency and an increase in expression of SLAM signaling factors characterizes sarcoidosis remission: a 4-year longitudinal study. Respiratory Research, 15(1), 91.

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Immunophenotyping by Flow Cytometry

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Immunostaining and subsequent flow cytometry were performed according to standard protocol prior to Dmab administration and 48-72 hours after, on peripheral blood samples. The antibodies used were CD45 (PerCP), CD14 (FITC), CD 23 (PE), CD 123 (PE), CD 4(FITC)/ CD 8(PE)/ CD 3(PerCP), CD 3(FITC)/ CD 16+56(PE)/ CD 45(PerCP)/ CD 19(APC)/ CD16 (PE) (BD Bioscience), as previously described[21 (link)]. Briefly, 100 μl of whole fresh blood were stained with the appropriate antibodies as instructed by the manufacturers for 30 min at RT. 2 ml of BD lysis buffer was added in order to lyse the erythrocytes and the samples were incubated for 10 min at RT. The samples were centrifuged at 500 xg and the supernatants were discarded. Pellets were washed once with serum-free PBS and centrifuged at 500 xg for 5 min. The final pellet was re-suspended in 0.5 ml serum-free PBS and the samples were immediately analyzed using FACs Calibur and Cell Quest software. 50,000 events were collected for each staining. The percentage of positive cells for each antibody was determined. The gating for each cell population has been previously described[21 (link)].

Kyrgidis A., Yavropoulou M.P., Zikos P., Lagoudaki R., Tilaveridis J, & Zouloumis L. (2020). Changes in peripheral monocyte populations 48-72 hours after subcutaneous denosumab administration in women with osteoporosis. Journal of Musculoskeletal & Neuronal Interactions, 20(3), 339-346.

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Multiparametric Flow Cytometry of SB Biopsy

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Four-colour FC of fresh SB biopsy samples was performed with FACSCalibur (Becton Dickinson, San Diego, California, USA) using a panel of antibodies: CD45, CD14, surface CD3, cytoplasmic CD3, CD4, CD5, CD7, CD8, CD16+56, CD19, CD103, CD13, CD25, HLA-DR, CD30, TCR-alpha/beta and TCR-gamma/delta (BD Bioscience). Data were analysed using Cellquest software (Becton Dickinson).

Hussein S., Gindin T., Lagana S.M., Arguelles-Grande C., Krishnareddy S., Alobeid B., Lewis S.K., Mansukhani M.M., Green P.H.R, & Bhagat G. (2018). Clonal T cell receptor gene rearrangements in coeliac disease: implications for diagnosing refractory coeliac disease. Journal of clinical pathology, 71(9).

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Peripheral Blood Lymphocyte Profiling

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We performed the analysis of peripheral blood lymphocyte populations by one laser three-color flow cytometry (BD Biosciences FACS Calibur, USA). One-hundred microliter of whole blood was obtained and stained with 20 μl of the monoclonal antibodies (CD3(fluorescein isothiocyanate (FITC)), CD4(FITC), CD8 (peridinin-chlorophyll protein complex (PerCP)), CD16 + 56(APC), and CD19 (phycoerythrin (PE)) (Beckton Dickinson, BD, USA)). Then, the samples were incubated in the dark for 15 min at room temperature.

Karaatmaca B., Cagdas D., Esenboga S., Erman B., Tan C., Turul Ozgur T., Boztug K., van der Burg M., Sanal O, & Tezcan I. (2024). Heterogeneity in RAG1 and RAG2 deficiency: 35 cases from a single-centre. Clinical and Experimental Immunology, 215(2), 160-176.

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Characterization of Immunological Profiles in CID

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The concentrations of Ig (immunoglobulin) G, IgA and IgM were determined with nephelometric method. T-cell subsets, B-cells and NK-cells were evaluated with flow cytometry using monoclonal antibodies (CD3, CD4, CD8, CD19, CD16/56; Becton Dickinson, San Jose, USA). The proliferation functions of T-cells were evaluated with using PHA (Phytohemagglutinin) as in vitro. When the PHA proliferation responses less than 10% of control it was accepted as decreased. Activity of ADA (adenosine deaminase) and PNP (purine nucleoside phosphorylase) enzymes were evaluated by tandem mass spectrometric screening with elevated level of ADA or PNP substrates [14 (link)]. The phenotypic classification of CID patients was performed based on absolute count of peripheral T^–, B^– and NK-cells.

Akar H.H., Patiroglu T., Hershfield M, & van der Burg M. (2016). Combined immunodeficiencies: twenty years experience from a single center in Turkey. Central-European Journal of Immunology, 41(1), 107-115.

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Peripheral Blood Lymphocyte Profiling

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The analysis of peripheral blood lymphocyte populations was performed by 6-color flow cytometry (Attune NxT, Thermo Fisher, USA), using 100 μl of whole blood stained with 20 μl of monoclonal antibodies with fluorescein isothiocyanate (FITC), phycoerythrin (PE), allophycocyanin (APC), or peridinin–chlorophyl–protein (PerCP)) against T and B subset markers (obtained from eBioscience, Thermo Fisher, USA) and incubated in the dark for 15 min at room temperature. For lymphocyte analysis, CD3(FITC), CD4(FITC), CD8(PE), CD16⁺56(PE), and CD19(PE) were used and for T and B lymphocyte subgroup analysis, CD4(FITC), CCR7(PE), CD31(PE), CD45RA (APC), and CD8(PerCP) (Beckton Dickinson, BD, USA) were used.

Solcà N.M., Bernasconi M.V, & Piffaretti J.C. (2000). Mechanism of Metronidazole Resistance in Helicobacter pylori: Comparison of the rdxA Gene Sequences in 30 Strains. Antimicrobial Agents and Chemotherapy, 44(8), 2207-2210.

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