The excised vessels were dehydrated with 40 % sucrose, embedded in OTC, and frozen at −70 °C. The IVC was sectioned into slices of 10 µm thickness with a cryotome (Leica, Mannheim, Germany). Specimens were washed in PBS and stained with hematoxylin and eosin (H&E). For immunohistochemical staining, slides were incubated with primary antibodies against TF, CD31, p65, phospho-GSK3β and myosin IIA at 4 °C overnight (dilution 1:100, 1:100, 1:50 1:300 and 1:50, respectively). Alexa Fluor® 488-conjugated Donkey Anti-Goat IgG (H+L) antibody (1:600) and Alexa Fluor® 594-conjugated Donkey Anti-Goat IgG (H+L) antibody (1:800) were used as secondary antibodies (Invitrogen, Carlsbad, CA, USA). The nuclei were stained with DAPI. Pathological changes in IVC were observed under fluorescence microscopy (Leica).
Alexa fluor 488 conjugated donkey anti goat igg h l antibody
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Alexa Fluor® 488-conjugated Donkey Anti-Goat IgG (H+L) antibody is a fluorescently labeled secondary antibody used to detect and visualize primary antibodies raised in goat. The Alexa Fluor® 488 dye, which emits green fluorescence, is conjugated to the donkey anti-goat IgG antibody. This product can be used in various immunodetection techniques, such as immunofluorescence and flow cytometry.
Automatically generated - may contain errors
2 protocols using alexa fluor 488 conjugated donkey anti goat igg h l antibody
1
Cryosectioning and Immunostaining of Inferior Vena Cava
2
Immunofluorescence Imaging of Cellular Targets
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After cell fixation and permeabilization, they were incubated overnight with primary antibody at 4 °C followed by Alexa Fluor® 488 conjugated Donkey Anti-Goat IgG (H + L) antibody (Invitrogen, Carlsbad, CA, USA) and/or Alexa Fluor® 594 Donkey Anti-Goat IgG (H + L) antibody (Invitrogen, Carlsbad, CA, USA) and DAPI (Beyotime Biotechnology, China). The images were taken using a confocal microscope (Olympus, Tokyo, Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!