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Power sybr green mater mix

Manufactured by Thermo Fisher Scientific
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Power SYBR Green Master Mix is a ready-to-use solution for real-time PCR amplification and detection. It contains SYBR Green I dye, DNA polymerase, dNTPs, and necessary reaction buffers.

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Lab products found in correlation

Trizol reagent, by Thermo Fisher Scientific (2 mentions) High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (2 mentions) Lightcycler 480 real time pcr system, by Roche (2 mentions) Rnase free dnase, by Thermo Fisher Scientific (2 mentions) Random primer, by Roche (1 mentions) Superscript 4 reverse transcriptase, by Thermo Fisher Scientific (1 mentions) Quick rna miniprep plus, by Zymo Research (1 mentions)

Close spelling variants of this product

SYBR Green (3216 citations) Power SYBR Green (542 citations) SYBR Green mix (375 citations) Power SYBR Green mix (69 citations) Power Sybr green pcr mater mix (2 citations)

3 protocols using power sybr green mater mix

1

Quantitative Real-Time PCR Assay for Gene Expression

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Total cellular RNA was extracted using the TRIzol reagent (Invitrogen) according to the manufacturer’s instruction. Contaminating DNA was digested by pre-treatment with RNase-free DNase (Ambion). Single strand cDNA synthesis was carried out using the high-capacity cDNA reverse transcription kit (Applied Biosystems) followed by standard PCR reactions. Real-time PCR reactions were performed using Power SYBR Green Mater Mix (Applied Biosystems) using a LightCycler® 480 Real-time PCR System (Roche). Gene expression was normalized to β-actin cDNA levels and calculated as a relative gene expression using 2−ΔΔCt method.
Oligonucleotide primers are as follows: Human MRPL18: forward 5’-CATCAG AATGGCAAGGTTGTG-3’ and reverse 5’-AAGTTGATTCCCGCCTCTAAG-3’; Mouse MRPL18: forward 5’-AGCAAAGGAAGATAGGGCAC-3’ and reverse 5’-ACA GACATTTCCAGAACCGC-3’; Human HSPA1A: forward 5’-TGTGTAACCCCATCA TCAGC-3’ and reverse 5’-TCTTGGAAAGGCCCCTAATC-3’; Mouse HSPA1A: forward 5’-TGGTGCAGTCCGACATGAAG-3’ and reverse 5’-GCTGAGAGTCGTTG AAGTAGGC-3’; Human β-actin: forward 5’-AGCCTCGCCTTTGCCGA-3’ and reverse 5’-GCGCGGCGATATCATCATC-3’; Mouse β-actin: forward 5’-TTGCTGACAGGA TGCAGAAG-3’ and reverse 5’-ACTCCTGCTTGCTGATCCACAT-3’. Quantitation of target genes of each fraction was normalized using the reference Firefly luciferase. Primers used are forward 5’-ATCCGGAAGCGACCAACGCC-3’ and reverse 5’-GTCG GGAAGACCTGCCACGC-3’.
Zhang X., Gao X., Coots R.A., Conn C.S., Liu B, & Qian S.B. (2015). Translational control of the cytosolic stress response by mitochondrial ribosomal protein L18. Nature structural & molecular biology, 22(5), 404-410.
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2

RNA Isolation and qRT-PCR Workflow

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Mouse tissues were homogenized using mortar, pestle, syringe and 28-gauge needle in DNA/RNA Shield Buffer provided by ZYMO Quick-RNA Miniprep Plus (R1057). RNA was isolated according to the manufacturer’s instructions. cDNA was synthesized using Superscript IV reverse transcriptase (Invitrogen) and random primers (Roche). Product from cDNA synthesis without RT enzyme was used as a negative control to confirm the absence of genomic DNA. For qRT-PCR assay, Power SYBR Green mater mix (Applied Biosystems) and primers in final concentration of 0.2μM were used on an ABI 7300 machine. Each sample was run in triplicate, and the mean value of triplicate plotted in graphs. 5ng of cDNA was used per reaction. For each primer set, reaction efficiency (E) was calculated using standard curve, and E−Ct value of each gene was normalized to geometric mean of E−Ct values of housekeeping genes. For mouse tissue samples, Nono and Rpl13a were used for normalization. Primers are listed in Supp. Table 2.
Juan A.M., Foong Y.H., Thorvaldsen J.L., Lan Y., Leu N.A., Rurik J.G., Li L., Krapp C., Rosier C.L., Epstein J.A, & Bartolomei M.S. (2022). Tissue-specific Grb10/Ddc insulator drives allelic architecture for cardiac development. Molecular cell, 82(19), 3613-3631.e7.
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3

Quantitative Real-Time PCR Assay for Gene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total cellular RNA was extracted using the TRIzol reagent (Invitrogen) according to the manufacturer’s instruction. Contaminating DNA was digested by pre-treatment with RNase-free DNase (Ambion). Single strand cDNA synthesis was carried out using the high-capacity cDNA reverse transcription kit (Applied Biosystems) followed by standard PCR reactions. Real-time PCR reactions were performed using Power SYBR Green Mater Mix (Applied Biosystems) using a LightCycler® 480 Real-time PCR System (Roche). Gene expression was normalized to β-actin cDNA levels and calculated as a relative gene expression using 2−ΔΔCt method.
Oligonucleotide primers are as follows: Human MRPL18: forward 5’-CATCAG AATGGCAAGGTTGTG-3’ and reverse 5’-AAGTTGATTCCCGCCTCTAAG-3’; Mouse MRPL18: forward 5’-AGCAAAGGAAGATAGGGCAC-3’ and reverse 5’-ACA GACATTTCCAGAACCGC-3’; Human HSPA1A: forward 5’-TGTGTAACCCCATCA TCAGC-3’ and reverse 5’-TCTTGGAAAGGCCCCTAATC-3’; Mouse HSPA1A: forward 5’-TGGTGCAGTCCGACATGAAG-3’ and reverse 5’-GCTGAGAGTCGTTG AAGTAGGC-3’; Human β-actin: forward 5’-AGCCTCGCCTTTGCCGA-3’ and reverse 5’-GCGCGGCGATATCATCATC-3’; Mouse β-actin: forward 5’-TTGCTGACAGGA TGCAGAAG-3’ and reverse 5’-ACTCCTGCTTGCTGATCCACAT-3’. Quantitation of target genes of each fraction was normalized using the reference Firefly luciferase. Primers used are forward 5’-ATCCGGAAGCGACCAACGCC-3’ and reverse 5’-GTCG GGAAGACCTGCCACGC-3’.
Zhang X., Gao X., Coots R.A., Conn C.S., Liu B, & Qian S.B. (2015). Translational control of the cytosolic stress response by mitochondrial ribosomal protein L18. Nature structural & molecular biology, 22(5), 404-410.
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