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Anti aflatoxin b1

Manufactured by Merck Group
Sourced in Germany

Anti-Aflatoxin B1 is a laboratory product used to detect the presence of aflatoxin B1 in various samples. Aflatoxin B1 is a carcinogenic mycotoxin produced by certain fungi. The core function of this product is to provide a reliable means of identifying and quantifying aflatoxin B1 levels in samples, such as food and agricultural commodities.

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2 protocols using anti aflatoxin b1

1

Anti-Aflatoxin B1 Detection Protocol

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Anti-aflatoxin B1, aflatoxin B1 (Fig. 1), thiourea, thioctic acid, tyramine, N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC) were obtained from Sigma–Aldrich (Steinheim,Germany), 1-dodecanethiol was obtained from Aldrich (Milwaukee, USA), . All other chemicals used were of analytical grade. All buffers were prepared from water treated with a MilliQ system from Millipore (Bedford MA, USA). This treated water is called MilliQ water in the rest of this paper. The buffers were filtered and degassed before use.
Samples from contaminated Brazilian nuts containing contaminated and non-contaminated nuts were kindly provided by Tahuamanu S.A. Company (Pando,Bolivia).
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2

Detecting Aflatoxin B1 in Nuts

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Anti-aflatoxin B1, aflatoxin B1, thioctic acid, N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC), sodium cyanoborohydride, bovine serum albumin (BSA) and ovalbumin were purchased from Sigma–Aldrich (Steinheim, Germany), 1-dodecanethiol was obtained from Aldrich (Milwaukee, USA). All other chemicals used were of analytical grade. All buffers were prepared with water treated via a MilliQ system (18 ΩM cm) (Bedford MA, USA), in the rest of this paper called MilliQ water. The buffers were filtered and degassed before use. Sample from Brazilian nuts containing contaminated and non-contaminated nuts were kindly provided by Tahuamanu S.A. Company (Pando, Bolivia).
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