Low molecular weight chitosan (448869 from sigma, molecular weight 50,000–190,000 Da, degree of deacetylation >75%), Polyethylene glycol (PEG) (20243.6, sigma average Molecular weight-1,305–1,595), formaldehyde, lysozyme, 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS), basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma Aldrich. Acetic acid and Heparin was supplied from Merk. Acetic acid, Heparin, 2-propanol, and xylene was supplied from Merk. VEGF, bFGF and ELISA kits were procured from R and D biosystems. Fetal bovine serum (FBS) was purchased from Pan America. DMEM and Live/dead assay kit was from Invitrogen.
Dmem and live dead assay kit
Manufactured by Thermo Fisher Scientific
DMEM is a widely used cell culture medium that provides essential nutrients and growth factors to support the growth and maintenance of a variety of cell types. The Live/Dead Assay Kit is a fluorescence-based method for determining the viability of cells in culture. It uses two fluorescent dyes to distinguish live and dead cells.
Automatically generated - may contain errors
Lab products found in correlation
Lysozyme, by Merck Group (2 mentions)
N hydroxysuccinimide, by Merck Group (2 mentions)
1 ethyl 3 3 dimethylaminopropyl carbodiimide, by Merck Group (2 mentions)
Acetic acid, by Merck Group (1 mentions)
2 propanol, by Merck Group (1 mentions)
Heparin, by Merck Group (1 mentions)
Formaldehyde, by Merck Group (1 mentions)
Chitosan, by Merck Group (1 mentions)
Polyethylene glycol peg, by Merck Group (1 mentions)
Vascular endothelial growth factor (vegf), by Merck Group (1 mentions)
Basic fibroblast growth factor (bfgf), by Merck Group (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
2 protocols using dmem and live dead assay kit
1
Chitosan-PEG Hydrogel for Angiogenic Growth Factor Delivery
2
Heparin-Crosslinked PLGA Hydrogel Delivery
Check if the same lab product or an alternative is used in the 5 most similar protocols
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PLGA (50:50, molecular weight 7,000–17,000 acid terminated), bFGF, VEGF, BSA, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS), MTT, and lysozyme were purchased from Sigma, heparin and DCM were from Merk, and VEGF and bFGF ELISA kits were procured from R and D Biosystems. FBS was purchased from Pan America, and DMEM and live/dead assay kit were from Invitrogen.
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