Spinothalamic tract neurons were labeled by a retrogradely transported dye that was injected into the thalamus. Two to 3-week-old mice were anesthetized with sodium pentobarbital (50 mg/kg, intraperitoneally) and placed on a rodent stereotactic apparatus fitted with a mouse adapter (Stoelting, Wood Dale, IL). A retrograde tracer, FAST-DiI (1 μL, 5% in ethanol, Invitrogen), was injected into the ventrobasal complex (VB) of the thalamus, using a micropipette attached to a 5 μL Hamilton syringe needle.24 (link),46 (link) For VB injection, the stereotactic coordinates41 were calculated and adjusted with respect to the bregma, using the ratio of the bregma-lambda distance of young mice to that of adult mice (4.5 mm). The coordinates of the injection site were 1.48 mm posterior from the bregma, 1.37 mm lateral from the mid-sagittal plane, and 3.3 mm deep from the surface. Three to 4 days were allowed for the dye to be transported to the lumbar spinal cord.
Mouse adapter
Manufactured by Stoelting
Sourced in United States
The Mouse Adapter is a device that allows users to connect a standard computer mouse to laboratory equipment for data input and control purposes. The adapter serves as an interface between the mouse and the equipment, enabling the user to navigate menus, select options, and input data using the familiar mouse interface.
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Lab products found in correlation
5 protocols using mouse adapter
1
Retrograde Labeling of Spinothalamic Neurons
2
Retrograde Tracing of Spinothalamic Neurons
3
Chronic Oxycontin Infusion in Mice
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Female dams were anesthetized at P95 with isoflurane (5% induction, 2% maintenance, 0.5 l/min) and placed in the mouse adapter (Stoelting, Wood Dale, IL, United States). Body temperature was maintained at 37°C using a heating pad. The dorsum of the back was shaved and a ∼1 cm horizontal incision was made below the scapulae with subsequent formation of a subcutaneous pocket. The Alzet pump was implanted and continuously infused with either Oxy or sterile 0.9% NaCl (Veh) over a period of 60 days. The pump duration allowed for adequate post-surgical recovery time, breeding, and administration of treatment through weaning of offspring at P21. In addition, the use of a subcutaneous pump limited unwanted maternal stress that can occur with daily injections.
4
Alzet Pump Implantation in Mice
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Mice were anesthetized with isoflurane and placed in the mouse adapter (Stoelting, Wood Dale, IL). A subcutaneous pocket was created in the dorsum and an Alzet pump was implanted. The cannula from the Brain Infusion Kit 3 was stereotactically placed into the left lateral ventricle at the following coordinates from Bregma: posterior 0.20 mm, left 0.8 mm, ventral 2.5 mm. The cannula was fixed to the skull using the Cyanoacrylate Adhesive (Rocky Hills, CT) and the incision was closed using 4–0 sutures. After completion of experiments, mice were euthanized with pentobarbital and underwent perfusion with PBS followed by 4% paraformaldehyde. Brains were removed, underwent a series of alcohol dehydrations, and were embedded in paraffin. Eight μm coronal sections were obtained and confirmed placement of the cannula within the left lateral ventricle.
5
Lateral Ventricular Infusion in Mice
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Mice were anesthetized using a mixture of ketamine (15 mg/kg) and xylazine (2.5 mg/kg) and placed in a stereotaxic frame equipped with a mouse adapter and ear bars (Stoelting, Chicago, USA). A stainless steel cannula (7 mm in length, outer diameter 0.5 mm, and inner diameter 0.25 mm) was then implanted in the lateral ventricle and fixed to the skull using dental cement. The following coordinates were used according to the mouse brain atlas [39] antero-posterior (AP) -0.3 mm; lateral (L) + 1 mm; dorsoventral (DV) -1 mm. Animals were then left to recover for a period of 7 days. In the infusion day, a stainless-steel injection micro-needle (outer diameter 0.25 mm) was connected through a polyethylene catheter to a 1000 μL Hamilton precision syringe and then lowered into the lateral cerebral ventricle (DV 2.4 mm). Five μL of a solution containing the different drugs were delivered using an infusion pump at 1 μL/min flow. Animals in the control group received equal volumes of vehicle. The needle was left in place for 1 additional min after each infusion [29, 40] .
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