The NP (powder) were pre-wetted by adding a drop of absolute ethanol. The NP were then taken up in dispersion liquid (H2O + 0.05% BSA) to a concentration of 2.56 mg/mL. These suspensions were sonicated using a 450 W Digital Sonifier (Branson, Danbury, CT, USA) with 10% of the maximum energy for 16 min according to the Nanogenotox protocol [32 ]. The particle size was determined using Nanoparticle Tracking Analysis (Nanosight, Amesbury, UK), which is based on the Brownian movement of the NP. Each suspension was measured five times, filtered through a 0.45-μm filter and measured again five times. The particle size in the filtered suspensions is shown. It is expressed as median (size, to match the peak in the size distribution), and as an average, both ± SD of the five measurements.
450 w digital sonifier
Manufactured by Emerson
Sourced in United States
The 450 W Digital Sonifier is a laboratory equipment designed for ultrasonic processing. It provides a digital interface for precise control and monitoring of the sonication process.
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Lab products found in correlation
3 protocols using 450 w digital sonifier
1
Characterization of Nanoparticle Suspensions
2
Ultrasonic Sonication Protocol for Water
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The results described below were obtained with a commercial Branson 450 W Digital Sonifier, operating at 20 kHz through a 230 mm long tapered Ti probe, with a ¼” diameter (6.4 mm-Ø) tip. Input power is entered as a percentage value, with a minimum of 10% and programmable in 1% increments. All data was collected from sonications of 2 s duration, initiated manually via a button on the front panel of the control console.
The horn was mounted such that the tip was submerged 25 ± 1 mm below the surface of de-ionised water, within a tank measuring 420 × 438 × 220 mm3,Fig. 1 . High-speed imaging, as described in §2.3, was conducted through glass windows embedded within the walls of the tank, Fig. 1 (b).![]()
The horn was mounted such that the tip was submerged 25 ± 1 mm below the surface of de-ionised water, within a tank measuring 420 × 438 × 220 mm3,
Schematic representations of the experimental arrangement (a) the ultrasonic horn and swPCD. (b) top-down view of the dual-perspective high-speed imaging configuration. The horn-tip is arrowed in both representations.
3
Polyurethane Nanocapsules via Inverse Miniemulsion
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Nanocapsules containing hydrophilic payloads were typically prepared through an in situ reaction with selected monomers at a droplet interface using the inverse miniemulsion process. For the synthesis of the polyurethane capsules, 83 mg (0.57 mmol) of 1,8-diaminooctane, 1.0 g of water, 1.0 mg of dye (rhodamine green or rhodamine 6G) or doxorubicin and 6.0 mg sodium chloride were added to 7 g of cyclohexane containing 200 mg of Hypermerª B246. For pre-emulsification, the reaction mixture was stirred at room temperature for 1 h at 1200 rpm. After that, the mini-emulsion was obtained by ultrasonication of the mixture using a Branson 450 W digital sonifier
(1/4" tip) for 3 min (30 s pulse; 30 s pause).
An equimolar amount of bis carbonate moiety with respect to the amino monomer was dissolved in 4 g of the cyclohexane-dichloromethane mixture. To this, a catalytic amount of TEA was added. The reaction mixture was added in a dropwise manner to the above mentioned mini-emulsion dispersion, and the resulting mixture was left for stirring at room temperature 24h. The reaction mixture was then passed through a paper filter to separate any bulk product formed from the particulate dispersion. The milky dispersions (containing nanocapsules) were then directly used for the reaction.
(1/4" tip) for 3 min (30 s pulse; 30 s pause).
An equimolar amount of bis carbonate moiety with respect to the amino monomer was dissolved in 4 g of the cyclohexane-dichloromethane mixture. To this, a catalytic amount of TEA was added. The reaction mixture was added in a dropwise manner to the above mentioned mini-emulsion dispersion, and the resulting mixture was left for stirring at room temperature 24h. The reaction mixture was then passed through a paper filter to separate any bulk product formed from the particulate dispersion. The milky dispersions (containing nanocapsules) were then directly used for the reaction.
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